{"product_id":"myc-wild-type-and-mutant-orf-cdna-lentivirus-bhv19400097","title":"MYC (Wild Type \u0026 Mutant) ORF cDNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eMYC is a basic helix-loop-helix leucine zipper transcription factor and a central regulator of cell growth, proliferation, metabolism, ribosome biogenesis, and apoptosis. By dimerizing with its partner MAX and binding E-box elements, MYC activates or represses thousands of target genes that coordinate the cell cycle and biosynthetic capacity. MYC protein levels are tightly controlled by phosphorylation-dependent degradation; phosphorylation at threonine 58 promotes its turnover. The T58A point mutation abolishes this signal, stabilizing the protein. MYC is one of the most frequently deregulated oncogenes in human cancer, where its overexpression or stabilization drives uncontrolled proliferation, making it a major focus of oncology research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe MYC (Wild Type \u0026amp; Mutant) ORF cDNA Lentivirus enables stable overexpression of the human or mouse MYC open reading frame in mammalian cells, available as wild-type and the stabilizing T58A mutant. A CMV or EF1a promoter drives the MYC ORF fused to a C-terminal V5 tag, with a fluorescent reporter (GFP or RFP) and antibiotic selection marker separated by a self-cleaving peptide for independent translation from a single transcript. The optimized third-generation vector provides high expression and reliable genome integration for rapid stable cell line generation. Applications include studying MYC-driven proliferation, metabolism, transformation, and the functional impact of T58A-mediated protein stabilization. Particles transduce difficult-to-transfect cells, including primary and thawed cells; cloning is sequence-verified and expression validated by transient transfection.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis ORF cDNA lentivirus enables stable overexpression of MYC (NCBI Accession: NM_002467, NM_010849) in mammalian cells via a third-generation, VSV-G pseudotyped delivery system. The ORF cDNA is fused to a C-terminal epitope tag (V5, Myc, or HA) and expressed under a strong constitutive promoter (CMV). Reporter and selection marker components (GFP, RFP; Blasticidin, Puromycin) are co-expressed via self-cleaving P2A peptides, enabling independent protein production without fusion-tag artifacts.\u003c\/p\u003e\n\u003cp\u003eUltra-purification by PEG precipitation and sucrose gradient centrifugation yields high-titer particles suitable for primary cells, suspension cultures, and stem cells. Stable polyclonal cell lines are established within 10–14 days by antibiotic selection or FACS sorting. For in vivo applications, the serum-free formulation and VSV-G envelope support direct administration or further concentration for stereotactic injection.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"CMV-MYC(T58A)-V5 \/ GFP\/none \/ 3x10^6","offer_id":53251620667757,"sku":"LCV-0002-18931","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-MYC-V5 \/ GFP\/none \/ 3x10^6","offer_id":53311260361069,"sku":"LCV-0002-18932","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV–Myc-V5 \/ GFP\/none \/ 3x10^6","offer_id":53311260393837,"sku":"LCV-0002-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-MYC-V5 \/ RFP\/Blasticidin \/ 3x10^6","offer_id":53311260426605,"sku":"LCV-0002-18933","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV–Myc(T58A)-V5 \/ GFP\/none \/ 3x10^6","offer_id":53311260459373,"sku":"LCV-0002-18930","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-Myc-Flag \/ none\/Blasticidin \/ 3x10^6","offer_id":53311260492141,"sku":"LCV-0002-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV–Myc-V5 \/ RFP\/none \/ 3x10^6","offer_id":53311260524909,"sku":"LCV-0002-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-Myc-Flag \/ none\/Puromycin \/ 3x10^6","offer_id":53311260557677,"sku":"LCV-0002-3S","price":595.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/myc-wild-type-and-mutant-orf-cdna-lentivirus-bhv19400097","provider":"BioHippo","version":"1.0","type":"link"}