{"product_id":"myl4-antibody-myosin-light-chain-4-bha17135632","title":"MYL4 Antibody \/ Myosin light chain 4","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eMYL4 Antibody \/ Myosin light chain 4 is a anti-MYL4 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e MYL4\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eMYL4 antibody detects Myosin light chain 4 (also known as Atrial light chain 1 or ALC-1), a regulatory myosin light chain predominantly expressed in cardiac atrial muscle and embryonic skeletal muscle. Encoded by the MYL4 gene on chromosome 17q21.32, this protein plays an essential role in actomyosin interactions that generate contractile force. Myosin light chain 4 binds to the neck region of myosin heavy chains and modulates ATPase activity, influencing contraction velocity and calcium sensitivity. The protein's phosphorylation status fine-tunes muscle contractility and adaptation to physiological demands.\u003cbr\u003e\u003cbr\u003eMYL4 is expressed abundantly in atrial cardiomyocytes, where it contributes to the high contraction frequency of the atria. During development, MYL4 is also expressed in ventricles and skeletal muscle before being downregulated postnatally, replaced by other myosin light chain isoforms. Re-expression of MYL4 in adult ventricles has been reported under pathological conditions such as hypertrophic cardiomyopathy, heart failure, and atrial fibrillation, suggesting a role in cardiac remodeling and disease adaptation. The protein forms part of the sarcomeric thick filament complex and interacts with myosin heavy chain alpha and beta isoforms to regulate actin-myosin cross-bridge cycling.\u003cbr\u003e\u003cbr\u003eThe MYL4 antibody is widely used in cardiovascular and muscle physiology research to study contractile protein composition, developmental regulation, and disease-associated remodeling. Western blot analysis typically identifies a 22 kilodalton band corresponding to MYL4, while immunohistochemistry reveals strong cytoplasmic staining in atrial tissue and developing muscle fibers. The antibody assists in assessing cardiac maturation, contractile diversity, and cellular hypertrophy mechanisms. Because MYL4 has been implicated in atrial fibrillation and abnormal calcium handling, its detection provides diagnostic and mechanistic insights into heart rhythm disorders.\u003cbr\u003e\u003cbr\u003eBeyond the heart, MYL4 contributes to cytoskeletal organization and mechanotransduction in non-muscle cells. It also interacts with actin-regulating kinases and phosphatases that modify contractile responses. In molecular studies, MYL4 serves as a marker of atrial differentiation and a target for evaluating cardiac-specific gene expression in regenerative models. The MYL4 antibody supplied by\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=MYL4 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=MYL4 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=MYL4 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/MYL4 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=MYL4+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047298228589,"sku":"FY12729","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_03fc694f-e211-4f5d-b05b-7acb9e0c0419.jpg?v=1782237031","url":"https:\/\/www.ebiohippo.com\/products\/myl4-antibody-myosin-light-chain-4-bha17135632","provider":"BioHippo","version":"1.0","type":"link"}