{"product_id":"myl6-antibody-myosin-light-chain-6-bha17136199","title":"MYL6 Antibody \/ Myosin light chain 6","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eMYL6 Antibody \/ Myosin light chain 6 is a anti-MYL6 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS) with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e MYL6\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eMYL6 antibody detects Myosin light chain 6, a regulatory component of the actin-myosin cytoskeleton that contributes to muscle contraction and cytoskeletal organization. Encoded by the MYL6 gene on chromosome 12q13.3, this protein belongs to the myosin regulatory light chain family and binds to the neck region of myosin heavy chains, stabilizing the lever arm that generates contractile force. MYL6 is expressed in both smooth and non-muscle cells, where it regulates motor activity during cell motility, cytokinesis, and tension maintenance in the actin cytoskeleton.\u003cbr\u003e\u003cbr\u003eMYL6 is one of two major isoforms produced by alternative splicing: a smooth muscle form (MYL6) and a non-muscle form (MYL6B). The smooth muscle isoform plays a key role in regulating calcium-sensitive contraction via interaction with myosin light chain kinase (MLCK) and myosin phosphatase, both of which control phosphorylation of the myosin regulatory light chain. The balance between phosphorylated and dephosphorylated MYL6 modulates smooth muscle tone and cell movement. Its structural domains include EF-hand calcium-binding motifs that enable conformational changes during contractile activation.\u003cbr\u003e\u003cbr\u003eBeyond its role in muscle contraction, MYL6 contributes to intracellular transport, cytokinetic ring formation, and endothelial barrier regulation. Alterations in myosin light chain phosphorylation have been linked to vascular diseases, asthma, and tumor metastasis. Overexpression or dysregulation of MYL6 has been observed in certain cancers and fibrotic disorders, reflecting its role in actomyosin tension and epithelial-to-mesenchymal transition (EMT). In cardiac tissue, MYL6 interacts with actin-associated proteins to influence sarcomere assembly and stress response signaling.\u003cbr\u003e\u003cbr\u003eImmunohistochemical staining using MYL6 antibody shows cytoplasmic localization in smooth muscle fibers, endothelial cells, and myofibroblasts. This antibody is widely used in research involving muscle physiology, cytoskeletal organization, and cellular contractility. MYL6 antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=MYL6 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=MYL6 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=MYL6 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/MYL6 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=MYL6+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047321231725,"sku":"FY13297","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_6bfae687-7726-4266-b1be-2a4003be9a6d.jpg?v=1782237094","url":"https:\/\/www.ebiohippo.com\/products\/myl6-antibody-myosin-light-chain-6-bha17136199","provider":"BioHippo","version":"1.0","type":"link"}