{"product_id":"myot-antibody-myotilin-bha17135770","title":"MYOT Antibody \/ Myotilin","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eMYOT Antibody \/ Myotilin is a anti-MYOT Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm, cell membrane.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e MYOT\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC\/IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eMYOT antibody detects Myotilin, a structural protein of the sarcomeric Z-disc that maintains muscle integrity and organization. Encoded by the MYOT gene on chromosome 5q31.2, this cytoskeletal protein binds alpha-actinin, filamin C, and other Z-disc components to stabilize actin filament cross-linking and support mechanical tension during muscle contraction. Myotilin plays a central role in maintaining sarcomere alignment, muscle elasticity, and myofibrillar assembly.\u003cbr\u003e\u003cbr\u003eStructurally, Myotilin is a 57 kilodalton cytoplasmic protein containing two immunoglobulin-like domains at its C-terminus that mediate interactions with alpha-actinin and filamin. Its N-terminal serine-rich region supports actin binding and cross-linking, anchoring thin filaments at the Z-disc. Myotilin is expressed predominantly in skeletal and cardiac muscle, where it acts as a scaffold linking actin filaments to structural and signaling molecules that maintain contractile function.\u003cbr\u003e\u003cbr\u003eThe MYOT antibody is widely used in muscle biology, myopathy, and cytoskeletal research to study sarcomere structure, actin filament organization, and myofibrillogenesis. Western blot analysis identifies a 57 kilodalton band corresponding to Myotilin, while immunohistochemistry shows distinct Z-disc staining in muscle fibers. This antibody supports investigations into muscle development, maintenance, and degenerative conditions affecting the sarcomere.\u003cbr\u003e\u003cbr\u003eMutations in MYOT cause various myofibrillar myopathies, including limb-girdle muscular dystrophy type 1A and spheroid body myopathy. These mutations lead to Myotilin aggregation, sarcomeric disorganization, and progressive muscle weakness. Altered MYOT expression has also been linked to cardiac remodeling and hypertrophy. The MYOT antibody provides a critical tool for examining these pathogenic mechanisms and evaluating Myotilin's interactions with actin-binding and signaling proteins.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=MYOT - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=MYOT - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=MYOT - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/MYOT - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=MYOT+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047304225133,"sku":"FY12868","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_82484ea4-e6e3-4cd3-aab9-38de6762b11b.jpg?v=1782237052","url":"https:\/\/www.ebiohippo.com\/products\/myot-antibody-myotilin-bha17135770","provider":"BioHippo","version":"1.0","type":"link"}