{"product_id":"naa30-antibody-n-alpha-acetyltransferase-30-nat12-bha17135863","title":"NAA30 Antibody \/ N-alpha-acetyltransferase 30 \/ NAT12","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNAA30 Antibody \/ N-alpha-acetyltransferase 30 \/ NAT12 is a anti-NAA30 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NAA30\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eNAA30 antibody detects N-alpha-acetyltransferase 30, also known as N-acetyltransferase 12 (NAT12), an enzyme responsible for N-terminal acetylation of nascent proteins. The UniProt recommended name is N-alpha-acetyltransferase 30 (NAA30). This enzyme is a catalytic component of the NatC N-terminal acetyltransferase complex, which transfers an acetyl group from acetyl-CoA to the alpha-amino group of specific target proteins. N-terminal acetylation is among the most common protein modifications in eukaryotes, influencing protein stability, localization, and complex formation.\u003cbr\u003e\u003cbr\u003eFunctionally, NAA30 antibody identifies a 362-amino-acid enzyme that acetylates proteins beginning with methionine followed by a hydrophobic residue, such as leucine, isoleucine, or phenylalanine. This modification often occurs co-translationally as nascent polypeptides emerge from the ribosome. NAA30 partners with auxiliary subunits NAA35 and NAA38 to form the NatC complex, ensuring substrate specificity and interaction with the ribosomal surface. Through this mechanism, NAA30 regulates key cellular processes including mitochondrial targeting, vesicular trafficking, and protein degradation.\u003cbr\u003e\u003cbr\u003eThe NAA30 gene is located on chromosome 3p21.31 and encodes an enzyme localized predominantly in the cytosol and on the cytoplasmic face of the endoplasmic reticulum. It exhibits acetyltransferase activity dependent on acetyl-CoA availability, linking its function to cellular metabolism. N-terminal acetylation catalyzed by NAA30 prevents degradation of proteins by shielding the N-terminus from ubiquitin-mediated turnover. Many NatC substrates are involved in vesicular transport, actin organization, and Golgi maintenance.\u003cbr\u003e\u003cbr\u003eDysregulation of NAA30 has been associated with several diseases. Loss of function leads to impaired mitochondrial integrity and developmental abnormalities. In cancer, altered NAA30 expression influences cell proliferation and apoptosis through acetylation of proteins that regulate the cell cycle and cytoskeleton. The enzyme also plays a role in stress adaptation by stabilizing proteins involved in autophagy and oxidative defense. Experimental depletion of NAA30 reduces acetylation of key regulators such as Arl3 and Grh12, leading to defects in vesicle trafficking and Golgi structure.\u003cbr\u003e\u003cbr\u003eNAA30 antibody is commonly used in protein biochemistry and molecular biology to examine acetyltransferase complex assembly, post-translational modification, and protein maturation. It supports applications in western blotting, immunocytochemistry, and enzyme activity assays to track NatC complex localization and expression. Because NAA30 acts early during protein synthesis, it is essential for maintaining the acetylation balance that governs protein lifespan and signaling dynamics.\u003cbr\u003e\u003cbr\u003eStructurally, NAA30 contains a conserved acetyltransferase domain that binds acetyl-CoA and donor substrates. Mutational analysis has identified residues critical for catalysis and substrate recognition.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NAA30 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NAA30 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NAA30 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NAA30 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NAA30+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047307305325,"sku":"FY12961","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_953436b2-ae93-4955-bf7d-621db4ddaa65.jpg?v=1782237060","url":"https:\/\/www.ebiohippo.com\/products\/naa30-antibody-n-alpha-acetyltransferase-30-nat12-bha17135863","provider":"BioHippo","version":"1.0","type":"link"}