{"product_id":"naa60-antibody-n-alpha-acetyltransferase-60-bha17136036","title":"NAA60 Antibody \/ N-alpha-acetyltransferase 60","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNAA60 Antibody \/ N-alpha-acetyltransferase 60 is a anti-NAA60 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Golgi apparatus.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NAA60\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC\/IF, IP, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eNAA60 antibody detects N-alpha-acetyltransferase 60, a Golgi-associated enzyme responsible for acetylating the N-termini of transmembrane and cytosolic proteins. The UniProt recommended name is N-alpha-acetyltransferase 60 (NAA60). This enzyme belongs to the GNAT (GCN5-related N-acetyltransferase) family and contributes to post-translational protein modification, stability, and membrane targeting.\u003cbr\u003e\u003cbr\u003eFunctionally, NAA60 antibody identifies a 242-amino-acid acetyltransferase localized to the cytosolic face of the Golgi apparatus. NAA60 catalyzes the transfer of an acetyl group from acetyl-CoA to protein N-termini, particularly targeting lysine residues within membrane-bound substrates. Its unique subcellular localization distinguishes it from other N-terminal acetyltransferases (NATs) acting in the cytosol or nucleus.\u003cbr\u003e\u003cbr\u003eThe NAA60 gene is located on chromosome 16p13.3 and is broadly expressed in mammalian tissues, with enrichment in epithelial and secretory cells. By modifying transmembrane proteins, NAA60 influences vesicle trafficking, protein sorting, and Golgi membrane organization. It also contributes to cell cycle progression and mitotic spindle stability.\u003cbr\u003e\u003cbr\u003ePathologically, altered NAA60 activity has been linked to cell proliferation abnormalities and tumorigenesis. Reduced NAA60 expression affects Golgi morphology and disrupts intracellular transport. Research using NAA60 antibody supports studies in protein acetylation, Golgi biology, and membrane-associated protein regulation.\u003cbr\u003e\u003cbr\u003eNAA60 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect acetyltransferases in cell lysates and subcellular fractions. \u003cbr\u003e\u003cbr\u003eStructurally, N-alpha-acetyltransferase 60 contains a conserved acetyl-CoA-binding motif and catalytic residues typical of GNAT enzymes. This antibody enables investigation of NAA60's functional role in post-translational regulation and Golgi-associated protein processing.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NAA60 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NAA60 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NAA60 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NAA60 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NAA60+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047313138029,"sku":"FY13134","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_ed2f8928-1dcb-4ca7-9181-cdeb8c53041e.jpg?v=1782237092","url":"https:\/\/www.ebiohippo.com\/products\/naa60-antibody-n-alpha-acetyltransferase-60-bha17136036","provider":"BioHippo","version":"1.0","type":"link"}