{"product_id":"nab2-antibody-ngfi-a-binding-protein-2-bha17135308","title":"NAB2 Antibody \/ NGFI-A-binding protein 2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNAB2 Antibody \/ NGFI-A-binding protein 2 is a anti-NAB2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NAB2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC\/IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eThe NAB2 antibody targets NGFI-A-binding protein 2, a transcriptional corepressor encoded by the NAB2 gene. This protein modulates gene expression by interacting with EGR (early growth response) family transcription factors, particularly EGR1 and EGR2, to regulate cell differentiation, proliferation, and nerve development. NGFI-A-binding protein 2 acts as both a transcriptional repressor and activator depending on context, influencing neuronal signaling, myelination, and growth factor response pathways. The NAB2 antibody enables specific detection of this multifunctional nuclear protein in studies of gene regulation and neurodevelopment.\u003cbr\u003e\u003cbr\u003eNGFI-A-binding protein 2 contains two conserved EGR-binding domains and a repression domain that interacts with the chromatin remodeling machinery. Through these domains, NAB2 forms complexes with EGR transcription factors to fine-tune expression of immediate-early genes involved in cellular differentiation. The NAB2 antibody allows visualization of these interactions in neural and non-neural tissues, facilitating studies on transcriptional control mechanisms that balance growth and differentiation. In the nervous system, NAB2 is essential for Schwann cell maturation and myelin formation, acting downstream of EGR2 signaling.\u003cbr\u003e\u003cbr\u003eMutations or dysregulation of NGFI-A-binding protein 2 have been linked to neuropathies and developmental disorders. NAB2 deficiency in animal models disrupts myelination and peripheral nerve function, underscoring its importance in glial differentiation. The NAB2 antibody supports investigations into these phenotypes by enabling detection of expression patterns during neural development and regeneration. It also serves as a valuable tool for examining NAB2's role in non-neuronal contexts, including cardiac hypertrophy, vascular remodeling, and hormonal signaling.\u003cbr\u003e\u003cbr\u003eNAB2 has gained attention in oncology due to the formation of NAB2-STAT6 gene fusions, which drive solitary fibrous tumors. The fusion protein combines the NAB2 repression domain with the STAT6 activation domain, leading to constitutive transcriptional activation. The NAB2 antibody aids in identifying expression changes associated with these fusion events, supporting tumor classification and mechanistic studies. NAB2's broader roles in transcriptional regulation and growth control make it a versatile subject for molecular and pathological research.\u003cbr\u003e\u003cbr\u003eThe NAB2 antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, yielding strong nuclear staining consistent with its transcriptional function.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NAB2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NAB2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NAB2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NAB2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NAB2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047286071661,"sku":"FY12405","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_8343589e-a16b-410f-b8c4-415c1e7560c0.jpg?v=1782236992","url":"https:\/\/www.ebiohippo.com\/products\/nab2-antibody-ngfi-a-binding-protein-2-bha17135308","provider":"BioHippo","version":"1.0","type":"link"}