{"product_id":"naglu-antibody-alpha-n-acetylglucosaminidase-bha17135765","title":"NAGLU Antibody \/ Alpha-N-acetylglucosaminidase","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNAGLU Antibody \/ Alpha-N-acetylglucosaminidase is a anti-NAGLU Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NAGLU\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eNAGLU antibody detects Alpha-N-acetylglucosaminidase, a lysosomal enzyme responsible for the degradation of heparan sulfate. Encoded by the NAGLU gene on chromosome 17q21.2, this hydrolase catalyzes the removal of N-acetylglucosamine residues from the non-reducing ends of heparan sulfate chains, playing a critical role in glycosaminoglycan catabolism. Proper function of NAGLU is essential for maintaining lysosomal homeostasis and preventing the accumulation of undegraded substrates.\u003cbr\u003e\u003cbr\u003eNAGLU is synthesized as a preproenzyme that undergoes proteolytic processing in the endoplasmic reticulum and Golgi before trafficking to lysosomes. The mature enzyme is approximately 83 kilodaltons and operates optimally at acidic pH. It functions in concert with other lysosomal hydrolases to complete stepwise degradation of heparan sulfate, ensuring normal turnover of extracellular matrix components and cell-surface proteoglycans.\u003cbr\u003e\u003cbr\u003eThe NAGLU antibody is widely used in enzymology, lysosomal biology, and metabolic disease research to study glycosaminoglycan metabolism, lysosomal trafficking, and enzyme replacement mechanisms. Western blot analysis identifies a band corresponding to the mature lysosomal form of NAGLU, while immunofluorescence shows vesicular staining overlapping with lysosomal markers such as LAMP1. This antibody enables precise detection of enzyme localization and expression changes in normal and diseased tissues.\u003cbr\u003e\u003cbr\u003eMutations in NAGLU cause mucopolysaccharidosis type IIIB (MPS IIIB), also known as Sanfilippo syndrome type B, a lysosomal storage disorder characterized by progressive neurodegeneration and systemic accumulation of heparan sulfate. The NAGLU antibody is a valuable tool for diagnosing and modeling this disease, assessing enzyme replacement efficacy, and monitoring lysosomal function in gene therapy studies.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NAGLU - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NAGLU - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NAGLU - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NAGLU - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NAGLU+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047304814957,"sku":"FY12863","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_a50ef1f7-fd38-4cdf-b174-ad7dde4f0e69.jpg?v=1782237050","url":"https:\/\/www.ebiohippo.com\/products\/naglu-antibody-alpha-n-acetylglucosaminidase-bha17135765","provider":"BioHippo","version":"1.0","type":"link"}