{"product_id":"nans-antibody-sialic-acid-synthase-bha17135265","title":"NANS Antibody \/ Sialic acid synthase","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNANS Antibody \/ Sialic acid synthase is a anti-NANS Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA with listed reactivity in Human, Rat. Reported localization: Cytoplasm.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NANS\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC\/IF, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eThe NANS antibody targets Sialic acid synthase, a cytosolic enzyme encoded by the NANS gene that catalyzes the formation of N-acetylneuraminic acid (Neu5Ac), the most abundant sialic acid in mammals. Sialic acids are terminal sugars found on glycoproteins and glycolipids, playing vital roles in cell-cell communication, pathogen recognition, and immune modulation. Sialic acid synthase converts N-acetylmannosamine-6-phosphate and phosphoenolpyruvate into N-acetylneuraminic acid-9-phosphate, which is later dephosphorylated to yield Neu5Ac. The NANS antibody enables precise detection of this enzyme, supporting research into glycosylation pathways, metabolic regulation, and genetic disease mechanisms.\u003cbr\u003e\u003cbr\u003eSialic acid synthase is widely expressed in tissues that actively produce glycoproteins, including liver, brain, and immune cells. The enzyme's activity is essential for the biosynthesis of sialylated glycoconjugates, which are critical for neuronal development and immune recognition. The NANS antibody allows researchers to monitor NANS expression levels and subcellular localization, facilitating studies that explore how sialic acid metabolism supports neural growth and synaptic function. In the developing brain, altered sialylation affects axonal targeting and synaptic plasticity, making this enzyme an important target in neurobiology.\u003cbr\u003e\u003cbr\u003eMutations in NANS cause sialic acid synthase deficiency, a rare autosomal recessive disorder characterized by skeletal dysplasia, developmental delay, and metabolic abnormalities. These mutations impair enzyme activity, leading to reduced sialic acid production and accumulation of precursors such as N-acetylmannosamine. The NANS antibody is an essential reagent for diagnosing and studying this deficiency, allowing direct measurement of protein levels and identification of loss-of-function variants. By detecting Sialic acid synthase in cellular and tissue models, researchers can examine how disrupted glycan biosynthesis contributes to disease phenotypes.\u003cbr\u003e\u003cbr\u003eBeyond human pathology, Sialic acid synthase plays roles in host-pathogen interactions. Many viruses and bacteria exploit sialic acid residues for attachment and immune evasion. The NANS antibody helps elucidate how modulation of sialic acid synthesis influences infection susceptibility and immune responses. Additionally, this enzyme's regulation under hypoxia and stress conditions highlights its connection to energy metabolism and cellular adaptation.\u003cbr\u003e\u003cbr\u003eThe NANS antibody is suitable for western blotting, immunofluorescence, and immunohistochemistry. It yields strong cytoplasmic staining consistent with Sialic acid synthase localization.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NANS - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NANS - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NANS - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NANS - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NANS+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047285317997,"sku":"FY12362","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_6e66f9ab-c110-4211-a6f3-d07b83abfd59.jpg?v=1782236989","url":"https:\/\/www.ebiohippo.com\/products\/nans-antibody-sialic-acid-synthase-bha17135265","provider":"BioHippo","version":"1.0","type":"link"}