{"product_id":"nap1l4-antibody-nucleosome-assembly-protein-1-like-4-bha17135935","title":"NAP1L4 Antibody \/ Nucleosome assembly protein 1-like 4","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNAP1L4 Antibody \/ Nucleosome assembly protein 1-like 4 is a anti-NAP1L4 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NAP1L4\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eNAP1L4 antibody detects Nucleosome assembly protein 1-like 4, a histone chaperone involved in nucleosome assembly, chromatin remodeling, and transcriptional regulation. The UniProt recommended name is Nucleosome assembly protein 1-like 4 (NAP1L4). This protein belongs to the NAP1 family, which mediates histone transport and deposition during DNA replication and repair.\u003cbr\u003e\u003cbr\u003eFunctionally, NAP1L4 antibody identifies a 391-amino-acid nuclear and cytoplasmic protein that facilitates the assembly of histone H2A-H2B dimers into nucleosomes. NAP1L4 acts as a molecular chaperone that prevents non-specific aggregation of histones, ensuring their proper deposition onto DNA. It also participates in transcriptional activation by assisting chromatin remodeling complexes in regulating nucleosome dynamics near promoters and enhancers.\u003cbr\u003e\u003cbr\u003eThe NAP1L4 gene is located on chromosome 11p15.4 and encodes a ubiquitously expressed protein found in dividing and differentiating cells. NAP1L4 associates with cell cycle regulators and transcription factors to coordinate chromatin accessibility and gene expression. Its function is particularly important during embryogenesis, where rapid chromatin remodeling supports differentiation and proliferation. In somatic cells, NAP1L4 contributes to DNA damage response and maintenance of chromatin integrity.\u003cbr\u003e\u003cbr\u003eBeyond histone assembly, NAP1L4 interacts with key signaling molecules and nuclear export proteins, linking chromatin organization to gene regulatory networks. Dysregulation of NAP1L4 expression has been observed in several cancers and developmental disorders, where altered nucleosome assembly can lead to epigenetic instability and aberrant transcription. Reduced NAP1L4 function has been associated with growth defects and impaired cell cycle progression in experimental models.\u003cbr\u003e\u003cbr\u003eNAP1L4 antibody is widely used in chromatin biology, transcriptional regulation, and cancer research. It is suitable for immunoblotting, immunoprecipitation, and immunofluorescence to detect NAP1L4 localization and expression. This antibody supports studies of histone dynamics, chromatin assembly, and gene regulation. In developmental research, it aids in exploring chromatin organization during early embryonic differentiation.\u003cbr\u003e\u003cbr\u003eStructurally, NAP1L4 contains acidic regions that bind histones electrostatically and domains that facilitate dimerization and complex formation. Its activity is regulated by phosphorylation and nuclear-cytoplasmic transport.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NAP1L4 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NAP1L4 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NAP1L4 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NAP1L4 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NAP1L4+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047310287213,"sku":"FY13033","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_e1395f3a-3b22-4bb2-8487-b62a5dad1d58.jpg?v=1782237083","url":"https:\/\/www.ebiohippo.com\/products\/nap1l4-antibody-nucleosome-assembly-protein-1-like-4-bha17135935","provider":"BioHippo","version":"1.0","type":"link"}