{"product_id":"naxe-antibody-nad-p-hx-epimerase-bha17135315","title":"NAXE Antibody \/ NAD(P)HX epimerase","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNAXE Antibody \/ NAD(P)HX epimerase is a anti-NAXE Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm (Mitochondria), Secreted.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NAXE\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eThe NAXE antibody targets NAD(P)HX epimerase, an enzyme encoded by the NAXE gene that catalyzes the interconversion of the R and S epimers of NADHX and NADPHX. These damaged forms of NADH and NADPH arise through hydration of nicotinamide cofactors under stress conditions. NAD(P)HX epimerase acts together with NAD(P)HX dehydratase to repair these inactive metabolites, maintaining cofactor homeostasis and protecting cells from metabolic imbalance. The NAXE antibody enables precise detection of this enzyme in studies of cofactor metabolism, mitochondrial function, and cellular stress response.\u003cbr\u003e\u003cbr\u003eNAD(P)HX epimerase localizes to both the cytosol and mitochondria, reflecting its role in multiple redox compartments. It is essential for the NAD(P)HX repair system, which safeguards the integrity of cellular redox reactions by regenerating functional NADH and NADPH. The NAXE antibody allows researchers to analyze this protective mechanism, revealing how cells maintain coenzyme pools under conditions of heat, oxidative, or metabolic stress. Loss of NAXE function leads to accumulation of toxic NADHX derivatives, disrupting energy metabolism and enzymatic activity.\u003cbr\u003e\u003cbr\u003eMutations in the NAXE gene cause a rare neurometabolic disorder characterized by recurrent encephalopathy and neurodegeneration, particularly following febrile illness. The NAXE antibody supports mechanistic studies into this disease, helping identify tissue-specific expression and enzyme deficiency in patient-derived samples. Defective NAD(P)HX epimerase activity compromises mitochondrial respiration and redox balance, underscoring its essential role in cellular survival.\u003cbr\u003e\u003cbr\u003eBeyond its role in repair metabolism, NAD(P)HX epimerase contributes to stress adaptation and antioxidant defense. It ensures a continuous supply of NAD(P)H required for reductive biosynthesis and detoxification pathways. The NAXE antibody supports quantitative analyses of enzyme regulation in models of oxidative stress, aging, and metabolic disorders. Dysregulation of NAD(P)HX repair enzymes has also been linked to impaired neuronal function and energy deficiency syndromes.\u003cbr\u003e\u003cbr\u003eThe NAXE antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, providing characteristic mitochondrial and cytosolic staining.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NAXE - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NAXE - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NAXE - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NAXE - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NAXE+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047289217389,"sku":"FY12412","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_8e2e6ed0-1189-49a9-bf84-a9253f7df11f.jpg?v=1782237022","url":"https:\/\/www.ebiohippo.com\/products\/naxe-antibody-nad-p-hx-epimerase-bha17135315","provider":"BioHippo","version":"1.0","type":"link"}