{"product_id":"ncapg-antibody-non-smc-condensin-i-complex-subunit-g-bha17135844","title":"NCAPG Antibody \/ Non-SMC condensin I complex subunit G","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNCAPG Antibody \/ Non-SMC condensin I complex subunit G is a anti-NCAPG Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Nuclear, cytoplasmic.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NCAPG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC\/IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eNCAPG antibody detects Non-SMC condensin I complex subunit G, an essential component of the condensin I complex that mediates chromosome condensation and segregation during mitosis. The UniProt recommended name is Non-SMC condensin I complex subunit G (NCAPG), with alternate names condensin complex subunit G, chromosome-associated protein G, and CAP-G. NCAPG functions as a regulatory subunit within the condensin complex, coordinating chromosome supercoiling and stabilization necessary for accurate chromosomal segregation.\u003cbr\u003e\u003cbr\u003eDuring cell division, NCAPG antibody identifies a protein critical for organizing chromatin architecture. NCAPG, together with SMC2, SMC4, NCAPH, and NCAPD2, composes condensin I, which compacts chromatin fibers into mitotic chromosomes. It binds ATP-dependent SMC core units and interacts with regulatory partners that modulate DNA topology and cohesion release. In addition to its structural role, NCAPG has been linked to transcriptional regulation, genomic stability, and cell proliferation pathways. Abnormal expression of NCAPG correlates with aneuploidy, mitotic defects, and tumor progression in various cancers, including hepatocellular carcinoma, glioblastoma, and lung cancer.\u003cbr\u003e\u003cbr\u003eThe NCAPG antibody is used to monitor cell cycle progression, particularly in the G2\/M phase where condensin activation occurs. Knockdown of NCAPG leads to chromosome segregation errors, DNA damage, and mitotic arrest. Studies also show NCAPG involvement in DNA repair and replication stress responses. The NCAPG gene is located on chromosome 4p15.32 and encodes a 1015-amino acid nuclear protein. NCAPG's expression peaks in proliferating cells and declines upon differentiation, making it a useful marker of mitotic activity.\u003cbr\u003e\u003cbr\u003eBeyond its canonical mitotic role, NCAPG contributes to chromatin reorganization during transcriptional activation and may interact with epigenetic regulators. Upregulation of NCAPG in cancer promotes oncogenic growth via activation of PI3K\/AKT and MAPK signaling pathways. High NCAPG levels are associated with poor prognosis and therapeutic resistance, suggesting it as a biomarker and target for anti-proliferative therapy. Researchers use NCAPG antibody for western blotting, immunostaining, and flow cytometry to study mitotic chromosome structure and cell cycle regulation.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NCAPG - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NCAPG - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NCAPG - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NCAPG - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NCAPG+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047306748269,"sku":"FY12942","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_7a015fea-7af3-44ee-9a8c-0602788619f5.jpg?v=1782237055","url":"https:\/\/www.ebiohippo.com\/products\/ncapg-antibody-non-smc-condensin-i-complex-subunit-g-bha17135844","provider":"BioHippo","version":"1.0","type":"link"}