{"product_id":"ncf1-antibody-neutrophil-cytosolic-factor-1-p47phox-bha17135245","title":"NCF1 Antibody \/ Neutrophil cytosolic factor 1 \/ p47phox","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNCF1 Antibody \/ Neutrophil cytosolic factor 1 \/ p47phox is a anti-NCF1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Flow cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasmic.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NCF1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e ELISA, FACS, IF, IHC, WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eThe NCF1 antibody targets Neutrophil cytosolic factor 1, a cytoplasmic component of the NADPH oxidase complex responsible for generating reactive oxygen species (ROS) during immune responses. Encoded by the NCF1 gene, this protein, also known as p47phox, acts as an organizer subunit that bridges cytosolic oxidase components with the membrane-bound catalytic core. The NCF1 antibody is a vital reagent for understanding how oxidative bursts are initiated in phagocytes and how these processes regulate host defense, inflammation, and redox signaling.\u003cbr\u003e\u003cbr\u003eNeutrophil cytosolic factor 1 becomes phosphorylated upon cellular activation, prompting its translocation to the plasma membrane where it associates with p22phox, gp91phox (CYBB), and other subunits to assemble the active NADPH oxidase complex. This enzymatic system produces superoxide radicals essential for microbial killing. The NCF1 antibody enables detection of this phosphorylation-dependent activation and helps identify conditions that modulate oxidase assembly.\u003cbr\u003e\u003cbr\u003eGenetic defects in NCF1 lead to chronic granulomatous disease (CGD), a primary immunodeficiency characterized by recurrent infections due to defective superoxide generation. In such patients, mutations often cause reduced or absent p47phox expression. Using the NCF1 antibody, researchers can distinguish functional versus nonfunctional protein forms, aiding in diagnostic and mechanistic studies of CGD. Beyond innate immunity, Neutrophil cytosolic factor 1 also contributes to redox-dependent signaling in endothelial and neuronal cells, highlighting its importance beyond phagocytes.\u003cbr\u003e\u003cbr\u003eThe NCF1 antibody is suitable for western blotting, immunoprecipitation, and flow cytometry, where it detects both total and phosphorylated protein forms. Its applications extend to studies examining oxidative stress, cell signaling cascades, and inflammatory pathway regulation. Because NADPH oxidase activity affects vascular tone, apoptosis, and cytokine production, NCF1 has broad physiological relevance. The antibody provides a reliable means to quantify expression changes under oxidative or inflammatory stress.\u003cbr\u003e\u003cbr\u003eIn oncology and cardiovascular research, aberrant activation of NCF1-containing complexes has been associated with chronic inflammation and tissue damage. Elevated expression may amplify ROS production, promoting DNA damage and tumorigenesis. Conversely, impaired function can weaken immune defense and tissue repair. The NCF1 antibody offered by \u003cbr\u003e\u003cbr\u003eBy providing consistent detection across multiple platforms, the NCF1 antibody remains an essential reagent for studying oxidative metabolism and immune cell function. Its use continues to expand from immunology into neurobiology and cancer biology, where understanding reactive oxygen mechanisms is increasingly central to therapeutic innovation.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NCF1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NCF1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NCF1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NCF1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NCF1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047284662637,"sku":"FY12342","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_ae389276-298e-47a1-af84-6cc246a72b38.jpg?v=1782236995","url":"https:\/\/www.ebiohippo.com\/products\/ncf1-antibody-neutrophil-cytosolic-factor-1-p47phox-bha17135245","provider":"BioHippo","version":"1.0","type":"link"}