{"product_id":"nci-h196-cell-bhc11101137","title":"NCI-H196 cell","description":"NCI-H196 is a small-cell lung cancer (SCLC) cell line used to study the mechanisms of cancer progression, chemotherapy resistance, and cellular responses to oxidative stress. Research involving NCI-H196 has demonstrated its sensitivity to the cytotoxic effects of pyrrolidine dithiocarbamate (PDTC), a pro-oxidant agent. PDTC induces S-phase cell cycle arrest and significantly reduces the viability of NCI-H196 cells in a dose-dependent manner. This cytotoxicity is attributed to the induction of oxidative stress, as evidenced by increased reactive oxygen species (ROS) and changes in the expression of oxidative stress-related genes. The addition of antioxidants like N-acetyl-L-cysteine (NAC) can effectively reverse PDTC-induced cytotoxicity, confirming the role of oxidative stress in cell death.\nFurther studies have shown that PDTC enhances the cytotoxicity of cisplatin, a first-line chemotherapy drug used for SCLC treatment. Combining low doses of cisplatin with non-toxic concentrations of PDTC leads to synergistic cytotoxicity in NCI-H196 cells. This combination therapy is believed to be effective due to PDTC’s downregulation of ATP7A, a copper efflux transporter associated with cisplatin resistance. By inhibiting ATP7A, PDTC may increase intracellular copper and sensitize NCI-H196 cells to cisplatin, highlighting its potential as an adjunct therapy for SCLC.\n\u003cp style=\"display:none\"\u003eSKU:BHC11101137\u003c\/p\u003e","brand":"Cytion","offers":[{"title":"1 cryovial","offer_id":52950218473837,"sku":"300390","price":550.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/NCI-H196_20_282_29_1920x1920_a7afa232-639b-432e-b0a5-78d9157802dc.jpg?v=1769069166","url":"https:\/\/www.ebiohippo.com\/products\/nci-h196-cell-bhc11101137","provider":"BioHippo","version":"1.0","type":"link"}