{"product_id":"nci-h446-cell-bhc11101136","title":"NCI-H446 cell","description":"This cell line was established in 1982 by D. Carney, A.F. Gazdar and associates from the pleural fluid of a patient with small cell cancer of the lung. The original tumor morphology was not characteristic of small cell lung cancer. The cell line is a variant small cell lung cancer in biochemistry and morphology, and expresses neuron specific enolase as well as the brain isoenzyme of creatine kinase. None of L-DOPA decarboxylase, bombesin, vasopressin, oxytocin or gastrin releasing peptide has been detected in the cell line. This cell line exhibits a 20-fold higher degree of c-myc DNA amplification and a 15-fold higher degree of c-myc RNA. The cell line was originally propagated in serum free RPMI 1640 medium supplemented with 10 nM of hydrocortisone, 5 microgram\/mL of insulin, 10 microgram\/mL of transferrin, 10 nM of 17-beta-estradiol, and 30 nM of sodium selenite. Transplantable tumors with non-typical small cell lung cancer histology can be formed by the cells.\n\u003cp style=\"display:none\"\u003eSKU:BHC11101136\u003c\/p\u003e","brand":"Cytion","offers":[{"title":"1 cryovial","offer_id":52950219161965,"sku":"305049","price":395.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/NCI-H446_20_281_29_1920x1920_62500070-f663-4e62-860b-0ddce8074344.jpg?v=1769069172","url":"https:\/\/www.ebiohippo.com\/products\/nci-h446-cell-bhc11101136","provider":"BioHippo","version":"1.0","type":"link"}