{"product_id":"ncln-antibody-nicalin-bha17135262","title":"NCLN Antibody \/ Nicalin","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNCLN Antibody \/ Nicalin is a anti-NCLN Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm (ER).\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NCLN\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC, IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eThe NCLN antibody targets Nicalin, a membrane protein encoded by the NCLN gene that functions as a component of the Nicalin-NOMO-TMEM147 complex. This complex regulates the maturation and assembly of membrane and secretory proteins within the endoplasmic reticulum (ER). Nicalin plays a vital role in early development, cell differentiation, and signaling processes associated with protein trafficking and folding. The NCLN antibody provides researchers with a highly specific reagent to explore how Nicalin contributes to ER homeostasis and membrane protein biosynthesis.\u003cbr\u003e\u003cbr\u003eNicalin is an integral membrane glycoprotein with several transmembrane domains that anchor it to the ER. It interacts with NOMO (Nodal modulator) and TMEM147 to form a multiprotein assembly that negatively regulates Nodal signaling, a pathway critical for embryonic patterning and left-right asymmetry. The NCLN antibody allows visualization of this protein complex and quantification of Nicalin expression in cultured cells and tissue samples, enabling insights into how ER-based regulatory systems influence developmental signaling.\u003cbr\u003e\u003cbr\u003eBeyond embryogenesis, Nicalin maintains normal ER function in adult cells by modulating protein assembly and trafficking. The NCLN antibody is particularly valuable for identifying changes in Nicalin expression during ER stress or unfolded protein response activation. These studies contribute to understanding how misfolded proteins accumulate in disease states such as neurodegeneration, metabolic disorders, and cancer. The localization of Nicalin at the ER membrane and its role in controlling protein export highlight its importance in maintaining proteostasis and cellular viability.\u003cbr\u003e\u003cbr\u003eRecent studies suggest that Nicalin may also regulate the biosynthesis of specific receptor and transporter proteins, linking it to neurotransmission and metabolic control. The NCLN antibody supports such investigations by enabling detection of Nicalin across various cell types, including neurons, epithelial cells, and hepatocytes. Its use in immunofluorescence and immunohistochemistry provides high-resolution imaging of ER organization and allows researchers to study how alterations in ER machinery affect overall cell physiology.\u003cbr\u003e\u003cbr\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NCLN - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NCLN - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NCLN - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NCLN - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NCLN+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047285285229,"sku":"FY12359","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_6819ddce-f811-4672-8d20-a06b94f757f9.jpg?v=1782237018","url":"https:\/\/www.ebiohippo.com\/products\/ncln-antibody-nicalin-bha17135262","provider":"BioHippo","version":"1.0","type":"link"}