{"product_id":"ncoa6-antibody-nuclear-receptor-coactivator-6-bha17136206","title":"NCOA6 Antibody \/ Nuclear receptor coactivator 6","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNCOA6 Antibody \/ Nuclear receptor coactivator 6 is a anti-NCOA6 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NCOA6\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC\/IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eNCOA6 antibody detects Nuclear receptor coactivator 6, a transcriptional coactivator that enhances the activity of nuclear hormone receptors and other transcription factors. Encoded by the NCOA6 gene on chromosome 20q12, this large nuclear protein plays a central role in gene expression regulation, chromatin remodeling, and hormone signaling. NCOA6, also known as ASC-2 (Activator of Steroid Receptor Coactivator-2), functions within multiprotein complexes that recruit histone acetyltransferases (HATs) and methyltransferases to promote transcriptional activation. Its modular structure includes LXXLL nuclear receptor-interaction motifs, a central activation domain, and regions that associate with chromatin modifiers such as CBP\/p300 and CARM1.\u003cbr\u003e\u003cbr\u003eNCOA6 acts as a transcriptional bridge connecting ligand-activated nuclear receptors-including estrogen receptor, thyroid hormone receptor, glucocorticoid receptor, and peroxisome proliferator-activated receptor gamma (PPARG)-to the general transcriptional machinery. Through these interactions, NCOA6 antibody detects a coactivator that is critical for endocrine signaling and metabolism regulation. NCOA6 also cooperates with non-receptor transcription factors such as E2F1, p53, and CREB-binding protein, extending its role beyond hormonal pathways. In metabolic tissues, it contributes to lipid metabolism, gluconeogenesis, and energy homeostasis through regulation of PGC-1alpha and FOXO1 signaling networks.\u003cbr\u003e\u003cbr\u003eStructurally, NCOA6 contains multiple protein-binding domains that facilitate interaction with chromatin-modifying enzymes and transcriptional complexes. It functions as a scaffold for the ASCOM complex (ASC-2 complex) that includes MLL family methyltransferases responsible for histone H3 lysine 4 (H3K4) methylation-a mark of active transcription. These modifications alter chromatin accessibility and promote RNA polymerase II recruitment. Alternative splicing of NCOA6 produces several isoforms with tissue-specific regulatory effects.\u003cbr\u003e\u003cbr\u003eNCOA6 is expressed ubiquitously but is enriched in liver, reproductive tissues, and endocrine organs. Dysregulation of NCOA6 has been linked to developmental abnormalities, reproductive disorders, and cancers including breast, prostate, and liver carcinoma. Overexpression promotes estrogen-dependent gene transcription and can contribute to hormone-sensitive tumor progression. Genetic studies have also implicated NCOA6 variants in metabolic syndrome, insulin resistance, and congenital heart defects, reflecting its broad physiological significance.\u003cbr\u003e\u003cbr\u003eAt the chromosomal level, 20q12 amplification encompassing NCOA6 has been identified in several tumor types. Functionally, NCOA6 acts as a coactivator of MYC and E2F transcription factors, enhancing cell proliferation and oncogenic transformation. Conversely, NCOA6 depletion impairs cell-cycle progression and induces apoptosis, indicating its importance for tumor cell survival. Disease-relevant pathways regulated by NCOA6 include oxidative stress response, estrogen signaling, and retinoic acid receptor pathways.\u003cbr\u003e\u003cbr\u003eImmunohistochemical staining using NCOA6 antibody shows nuclear localization in hepatocytes, endocrine gland cells, and epithelial tissues. The antibody is a valuable tool for studies of transcriptional regulation, hormone receptor signaling, and chromatin remodeling. NCOA6 antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NCOA6 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NCOA6 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NCOA6 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NCOA6 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NCOA6+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047321362797,"sku":"FY13304","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_2f436d00-22e4-4901-a2f3-a9f10fff6440.jpg?v=1782237111","url":"https:\/\/www.ebiohippo.com\/products\/ncoa6-antibody-nuclear-receptor-coactivator-6-bha17136206","provider":"BioHippo","version":"1.0","type":"link"}