{"product_id":"ndst1-antibody-n-deacetylase-n-sulfotransferase-1-bha17135520","title":"NDST1 Antibody \/ N-deacetylase\/N-sulfotransferase 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNDST1 Antibody \/ N-deacetylase\/N-sulfotransferase 1 is a anti-NDST1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Western blot (WB) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NDST1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e ELISA, WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eNDST1 antibody detects N-deacetylase\/N-sulfotransferase 1, an essential enzyme involved in the biosynthesis of heparan sulfate, a glycosaminoglycan that modulates cell signaling, adhesion, and extracellular matrix organization. NDST1 catalyzes two critical reactions-N-deacetylation and N-sulfation of glucosamine residues-forming the sulfated domains that give heparan sulfate its functional specificity. The NDST1 antibody is widely used in glycobiology, developmental, and cancer research to study proteoglycan biosynthesis, cell-matrix interactions, and signaling regulation.\u003cbr\u003e\u003cbr\u003eNDST1 is encoded by the NDST1 gene located on human chromosome 5q33.1. The protein is approximately 882 amino acids long and resides in the lumen of the Golgi apparatus, where it acts early in heparan sulfate chain modification. NDST1 belongs to the heparan sulfate modification enzyme family, working in concert with other sulfotransferases and epimerases to establish specific sulfation patterns that control the binding affinity of heparan sulfate to growth factors, morphogens, and cytokines.\u003cbr\u003e\u003cbr\u003eThe NDST1 antibody detects a 100 kilodalton band by western blot and demonstrates perinuclear Golgi localization under immunofluorescence microscopy. NDST1 activity is essential for embryonic development, vascular formation, and neural patterning. Through its modification of heparan sulfate, NDST1 regulates key signaling pathways including FGF, Wnt, Hedgehog, and BMP. Loss or mutation of NDST1 disrupts heparan sulfate composition, leading to developmental abnormalities and impaired growth factor signaling.\u003cbr\u003e\u003cbr\u003eIn cancer, NDST1 expression is often dysregulated, resulting in altered extracellular matrix structure and aberrant cell signaling. Overexpression enhances tumor invasiveness by modifying cell-surface heparan sulfate, while reduced NDST1 activity interferes with angiogenesis. It also contributes to inflammation by modulating chemokine gradients and leukocyte migration.\u003cbr\u003e\u003cbr\u003eBecause NDST1 determines the sulfation code of heparan sulfate, it serves as a critical regulator of cell communication and extracellular architecture.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NDST1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NDST1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NDST1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NDST1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NDST1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047294787949,"sku":"FY12617","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_515d44e8-b445-40d9-97dc-f93de0bf7c5d.jpg?v=1782237028","url":"https:\/\/www.ebiohippo.com\/products\/ndst1-antibody-n-deacetylase-n-sulfotransferase-1-bha17135520","provider":"BioHippo","version":"1.0","type":"link"}