{"product_id":"ndufa1-antibody-nadh-dehydrogenase-ubiquinone-1-alpha-subcomplex-subunit-1-bha17135685","title":"NDUFA1 Antibody \/ NADH dehydrogenase ubiquinone 1 alpha subcomplex subunit 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNDUFA1 Antibody \/ NADH dehydrogenase ubiquinone 1 alpha subcomplex subunit 1 is a anti-NDUFA1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm (Mitochondria).\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NDUFA1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eNDUFA1 antibody detects NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1, a core accessory protein of mitochondrial Complex I that contributes to oxidative phosphorylation and energy metabolism. Encoded by the NDUFA1 gene on the X chromosome (Xp11.3), this protein is one of approximately 45 subunits forming NADH:ubiquinone oxidoreductase, the first enzyme in the mitochondrial electron transport chain. NDUFA1 helps anchor hydrophobic membrane components of Complex I to its hydrophilic catalytic core, maintaining structural stability and supporting efficient electron transfer from NADH to ubiquinone.\u003cbr\u003e\u003cbr\u003eNDUFA1 is a small, highly conserved protein localized to the mitochondrial inner membrane. It associates with other accessory subunits of the alpha subcomplex, including NDUFA2 and NDUFA3, and is essential for the assembly and functional integrity of Complex I. By stabilizing the membrane domain, NDUFA1 ensures proper coupling of proton translocation with electron transfer, thereby contributing to the mitochondrial proton gradient and ATP synthesis. Mutations in NDUFA1 can impair Complex I activity, leading to mitochondrial encephalomyopathy, lactic acidosis, and neurodegenerative diseases.\u003cbr\u003e\u003cbr\u003eThe NDUFA1 antibody is widely used in mitochondrial biology, metabolism, and neurodegeneration research to study electron transport chain organization and oxidative phosphorylation. Western blot analysis identifies a 7 kilodalton band corresponding to NDUFA1, while immunofluorescence reveals mitochondrial localization colocalizing with markers such as TOM20 and COX IV. This antibody allows characterization of mitochondrial respiratory complexes under physiological and disease conditions.\u003cbr\u003e\u003cbr\u003eDefects in Complex I assembly caused by NDUFA1 dysfunction contribute to reduced ATP production and excessive reactive oxygen species generation, triggering oxidative stress and apoptosis. The NDUFA1 antibody provides a key tool for investigating mitochondrial respiratory disorders, metabolic adaptation, and redox regulation.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NDUFA1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NDUFA1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NDUFA1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NDUFA1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NDUFA1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047299998061,"sku":"FY12782","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_0bd0cc97-3159-4801-b3b3-c4e719ee608c.jpg?v=1782237060","url":"https:\/\/www.ebiohippo.com\/products\/ndufa1-antibody-nadh-dehydrogenase-ubiquinone-1-alpha-subcomplex-subunit-1-bha17135685","provider":"BioHippo","version":"1.0","type":"link"}