{"product_id":"ndufab1-antibody-nadh-dehydrogenase-ubiquinone-1-alpha-subcomplex-subunit-1-bha17135716","title":"NDUFAB1 Antibody \/ NADH dehydrogenase ubiquinone 1 alpha subcomplex subunit 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNDUFAB1 Antibody \/ NADH dehydrogenase ubiquinone 1 alpha subcomplex subunit 1 is a anti-NDUFAB1 Rabbit antibody Recombinant Rabbit Monoclonal clone 30N94 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm (Mitochondria).\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NDUFAB1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 30N94, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, IP, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eNDUFAB1 antibody detects NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1, encoded by the NDUFAB1 gene. Other identifiers include NADH dehydrogenase 1 alpha subcomplex protein 1, acyl carrier protein NDUFAB1, mitochondrial complex I alpha subunit 9, and human homolog of E. coli acyl carrier protein. NDUFAB1 is a mitochondrial matrix protein that plays dual roles in respiratory chain function and fatty acid biosynthesis. As part of NADH:ubiquinone oxidoreductase (complex I), it participates in the first step of the mitochondrial electron transport chain, transferring electrons from NADH to ubiquinone and contributing to proton pumping across the inner mitochondrial membrane. In addition, NDUFAB1 acts as a mitochondrial acyl carrier protein involved in fatty acid and lipoic acid synthesis, integrating energy metabolism with biosynthetic pathways.\u003cbr\u003e\u003cbr\u003eNDUFAB1 antibody is widely applied in mitochondrial biology, metabolism, and disease research. Complex I is the largest enzyme complex of the respiratory chain, consisting of over 40 subunits, and NDUFAB1 provides both structural stability and accessory enzymatic functions. By detecting NDUFAB1, researchers can study how mitochondrial respiration couples with biosynthetic pathways to maintain cellular energy homeostasis. The dual functionality of NDUFAB1 highlights its importance beyond electron transport, as it also influences cofactor assembly and post translational modification of metabolic enzymes.\u003cbr\u003e\u003cbr\u003eApplications of NDUFAB1 antibody include western blotting, immunohistochemistry, immunofluorescence, and ELISA. Western blotting detects NDUFAB1 protein in mitochondrial fractions, immunohistochemistry maps tissue expression in high energy organs such as heart, brain, and skeletal muscle, and immunofluorescence highlights its localization in mitochondria. These methods allow researchers to connect NDUFAB1 biology to mitochondrial function at both cellular and tissue levels.\u003cbr\u003e\u003cbr\u003eDysfunction of NDUFAB1 or complex I assembly leads to mitochondrial disease. Deficiencies manifest as metabolic syndromes with lactic acidosis, muscle weakness, and neurodegeneration. Altered NDUFAB1 activity has been associated with Leigh syndrome, Parkinson disease, and other mitochondrial disorders. By applying NDUFAB1 antibody, scientists can study how complex I defects contribute to disease progression and explore NDUFAB1 as a diagnostic or therapeutic biomarker.\u003cbr\u003e\u003cbr\u003eNDUFAB1 also participates in mitochondrial fatty acid synthesis. This pathway produces acyl chains used for lipoic acid synthesis, essential for pyruvate dehydrogenase and other enzyme complexes. By acting as a mitochondrial acyl carrier protein, NDUFAB1 integrates respiratory function with biosynthetic activity. Detection with antibody based assays helps researchers dissect how mitochondria coordinate catabolism and anabolism.\u003cbr\u003e\u003cbr\u003eIn cancer research, mitochondrial reprogramming is a hallmark of tumor cells. NDUFAB1 expression influences oxidative phosphorylation and biosynthetic balance, impacting proliferation and survival. Increased NDUFAB1 expression has been observed in some cancers, while reduced activity impairs energy production and cell viability. The antibody therefore provides a tool for studying mitochondrial metabolism in cancer biology.\u003cbr\u003e\u003cbr\u003eBeyond disease, NDUFAB1 is studied in physiology, including muscle energetics, neuronal metabolism, and aging. Mitochondrial complex I function declines with age, and NDUFAB1 expression correlates with mitochondrial health. Antibody detection allows evaluation of how this subunit contributes to aging related metabolic changes.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NDUFAB1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NDUFAB1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NDUFAB1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NDUFAB1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NDUFAB1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047302488429,"sku":"FY12814","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_ba48fb21-82a7-4c8d-b701-94f367cef7c3.jpg?v=1782237039","url":"https:\/\/www.ebiohippo.com\/products\/ndufab1-antibody-nadh-dehydrogenase-ubiquinone-1-alpha-subcomplex-subunit-1-bha17135716","provider":"BioHippo","version":"1.0","type":"link"}