{"product_id":"ndufb11-antibody-nadh-dehydrogenase-ubiquinone-1-beta-subcomplex-subunit-11-bha17135767","title":"NDUFB11 Antibody \/ NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNDUFB11 Antibody \/ NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11 is a anti-NDUFB11 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm (Mitochondria).\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NDUFB11\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC\/IF\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eNDUFB11 antibody detects NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, a small integral membrane protein of the mitochondrial inner membrane essential for oxidative phosphorylation. Encoded by the NDUFB11 gene on chromosome Xp11.23, this protein is a subunit of mitochondrial Complex I (NADH:ubiquinone oxidoreductase), the first and largest enzyme complex of the electron transport chain. NDUFB11 contributes to electron transfer from NADH to ubiquinone, driving proton pumping and ATP synthesis. Its stability is vital for the assembly and structural integrity of Complex I, which contains over 40 subunits in mammalian cells.\u003cbr\u003e\u003cbr\u003eStructurally, NDUFB11 is a highly conserved, hydrophobic protein of approximately 17 kilodaltons, localized to the mitochondrial inner membrane with one transmembrane helix. It anchors and stabilizes neighboring subunits within the peripheral arm of Complex I, enabling efficient electron flow and coupling of redox reactions to proton translocation. Loss or mutation of NDUFB11 disrupts complex assembly, leading to impaired respiratory chain activity and reduced ATP generation.\u003cbr\u003e\u003cbr\u003eThe NDUFB11 antibody is widely used in mitochondrial biology, metabolic disease, and bioenergetics research to study oxidative phosphorylation and Complex I organization. Western blot analysis detects a 17 kilodalton band corresponding to NDUFB11, while immunofluorescence reveals characteristic mitochondrial localization that colocalizes with markers such as COX IV. This antibody provides a reliable means of evaluating mitochondrial function, biogenesis, and assembly defects in various models.\u003cbr\u003e\u003cbr\u003ePathogenic variants in NDUFB11 cause X-linked mitochondrial Complex I deficiency, often presenting as hypertrophic cardiomyopathy, encephalopathy, or lactic acidosis. Altered NDUFB11 expression has also been observed in cancers and neurodegenerative disorders, where mitochondrial dysfunction contributes to disease progression. The NDUFB11 antibody enables detection of expression changes, assessment of mitochondrial health, and verification of respiratory chain assembly status.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NDUFB11 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NDUFB11 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NDUFB11 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NDUFB11 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NDUFB11+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047304126829,"sku":"FY12865","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_35a5c32e-ae3a-4c26-95e7-34e3ee5c6804.jpg?v=1782237043","url":"https:\/\/www.ebiohippo.com\/products\/ndufb11-antibody-nadh-dehydrogenase-ubiquinone-1-beta-subcomplex-subunit-11-bha17135767","provider":"BioHippo","version":"1.0","type":"link"}