{"product_id":"ndufs1-antibody-bha17136356","title":"NDUFS1 Antibody","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNDUFS1 Antibody is a anti-NDUFS1 Rabbit antibody Polyclonal (rabbit origin) supplied in Purified format. Recommended for workflows such as Western blot (WB), IHC-P, Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NDUFS1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC-P, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eNDUFS1 (NADH:ubiquinone oxidoreductase core subunit S1) is a vital component of mitochondrial complex I, the largest enzyme of the respiratory chain. This nuclear-encoded protein serves as a core subunit of the iron-sulfur protein (ISP) fraction, where it plays an essential role in electron transfer from NADH to ubiquinone. By facilitating the first step in oxidative phosphorylation, NDUFS1 directly contributes to ATP production, making it crucial for cellular energy metabolism. Research utilizing an NDUFS1 antibody has highlighted the importance of this protein in maintaining mitochondrial integrity and function.    \u003cbr\u003e\u003cbr\u003e  Mutations in the NDUFS1 gene are associated with mitochondrial complex I deficiencies, a leading cause of numerous mitochondrial disorders. These conditions often present with neurodegenerative features, metabolic dysfunction, and progressive encephalopathy. Studies employing an NDUFS1 antibody have provided valuable insights into how loss of function impacts respiratory chain activity and contributes to disease pathology.    \u003cbr\u003e\u003cbr\u003e  Beyond inherited disorders, dysregulation of NDUFS1 has been implicated in aging, neurodegenerative diseases such as Parkinson's, and ischemic injury. Because mitochondrial dysfunction is a common hallmark of these conditions, NDUFS1 remains an attractive marker for evaluating mitochondrial activity in research models. Use of an NDUFS1 antibody allows for detailed detection in techniques including western blot, immunohistochemistry, and immunofluorescence.    \u003cbr\u003e\u003cbr\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NDUFS1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NDUFS1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NDUFS1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NDUFS1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NDUFS1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047326343533,"sku":"RQ8938","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_5f7bd650-43a8-41cb-aed4-eb03afea8f28.jpg?v=1772019451","url":"https:\/\/www.ebiohippo.com\/products\/ndufs1-antibody-bha17136356","provider":"BioHippo","version":"1.0","type":"link"}