{"product_id":"ndufs3-antibody-nadh-dehydrogenase-ubiquinone-iron-sulfur-protein-3-bha17135291","title":"NDUFS3 Antibody \/ NADH dehydrogenase ubiquinone iron-sulfur protein 3","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNDUFS3 Antibody \/ NADH dehydrogenase ubiquinone iron-sulfur protein 3 is a anti-NDUFS3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm (Mitochondria).\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NDUFS3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eThe NDUFS3 antibody targets NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, a core subunit of mitochondrial Complex I encoded by the NDUFS3 gene. This protein is an essential component of the oxidative phosphorylation (OXPHOS) system that catalyzes electron transfer from NADH to ubiquinone, driving ATP synthesis. NADH dehydrogenase [ubiquinone] iron-sulfur protein 3 contributes to the assembly and stabilization of Complex I and is indispensable for mitochondrial energy metabolism. The NDUFS3 antibody enables researchers to assess mitochondrial function and respiratory chain integrity under physiological and disease conditions.\u003cbr\u003e\u003cbr\u003eComplex I, the largest enzyme complex of the electron transport chain, consists of more than 40 subunits organized into functional modules. NDUFS3 forms part of the peripheral arm that houses redox centers responsible for electron transfer. It interacts with other core subunits, including NDUFS2 and NDUFS7, during the assembly of catalytic intermediates. The NDUFS3 antibody supports detailed examination of these interactions, helping to elucidate how mitochondrial protein complexes coordinate ATP production and energy regulation.\u003cbr\u003e\u003cbr\u003eDefects in NDUFS3 are associated with mitochondrial complex I deficiency, one of the most common causes of inherited metabolic disorders. Mutations can result in severe encephalomyopathy, cardiomyopathy, or Leigh syndrome, characterized by impaired oxidative phosphorylation and reduced ATP generation. The NDUFS3 antibody is a critical tool for studying such pathologies, allowing detection of expression defects and assessing the impact of genetic variants on mitochondrial function. Immunoblotting with this antibody reveals the presence and stability of Complex I subunits in patient-derived fibroblasts and model organisms.\u003cbr\u003e\u003cbr\u003eIn addition to its structural role, NADH dehydrogenase [ubiquinone] iron-sulfur protein 3 influences cellular redox balance and reactive oxygen species (ROS) generation. Dysregulation of Complex I components can contribute to oxidative stress, aging, and neurodegenerative diseases such as Parkinson's and Alzheimer's. The NDUFS3 antibody supports investigation of these mechanisms by enabling visualization of mitochondrial protein distribution and expression levels in tissues under oxidative stress or toxin exposure.\u003cbr\u003e\u003cbr\u003eThe NDUFS3 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry. It yields distinct mitochondrial staining, reflecting its subcellular localization.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NDUFS3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NDUFS3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NDUFS3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NDUFS3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NDUFS3+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047287742829,"sku":"FY12388","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_aa2613a0-221f-44a3-ab48-5bdb3ed893e8.jpg?v=1782237005","url":"https:\/\/www.ebiohippo.com\/products\/ndufs3-antibody-nadh-dehydrogenase-ubiquinone-iron-sulfur-protein-3-bha17135291","provider":"BioHippo","version":"1.0","type":"link"}