{"product_id":"nefm-antibody-nf-m-neurofilament-medium-bha17135958","title":"NEFM Antibody \/ NF-M \/ Neurofilament medium","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNEFM Antibody \/ NF-M \/ Neurofilament medium is a anti-NEFM Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm, cytoskeleton.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NEFM\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, IF, IP, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eNEFM antibody detects Neurofilament medium polypeptide, also known as NF-M, a critical intermediate filament protein that maintains neuronal structure and axonal integrity. The UniProt recommended name is Neurofilament medium polypeptide (NEFM). This protein is one of the three major neurofilament subunits-light (NEFL), medium (NEFM), and heavy (NEFH)-that assemble into heteropolymers forming the neuronal cytoskeleton.\u003cbr\u003e\u003cbr\u003eFunctionally, NEFM antibody identifies a 916-amino-acid cytoskeletal protein containing an alpha-helical coiled-coil rod domain and an extensive C-terminal tail rich in phosphorylation sites. NEFM contributes to the structural scaffolding of axons, determining their caliber and conduction velocity. Its phosphorylation regulates filament spacing and interaction with other cytoskeletal components, thereby influencing axonal transport and mechanical stability.\u003cbr\u003e\u003cbr\u003eThe NEFM gene is located on chromosome 8p21.2 and is highly expressed in neurons of the central and peripheral nervous systems. NEFM is particularly abundant in large myelinated axons, where it associates with microtubules and neurofilament light and heavy chains to form cross-linked filamentous arrays. During development, NEFM expression increases as neurons mature, supporting long-range axonal projection and signal transmission.\u003cbr\u003e\u003cbr\u003eIn pathology, NEFM serves as a biomarker of axonal injury and neurodegeneration. Elevated NEFM levels are detected in cerebrospinal fluid and plasma of patients with amyotrophic lateral sclerosis (ALS), Alzheimer's disease, and traumatic brain injury, reflecting axonal breakdown. In experimental models, altered NEFM phosphorylation contributes to impaired axonal transport and neurofilament aggregation, features commonly seen in neurodegenerative diseases.\u003cbr\u003e\u003cbr\u003eNEFM antibody is widely used in neuroscience, neuropathology, and cytoskeletal biology research. It is suitable for immunohistochemistry, immunofluorescence, and western blotting to detect NF-M localization and integrity in neuronal tissues. This antibody supports studies of axonal structure, neurofilament organization, and neural injury responses. In translational studies, NEFM detection provides a molecular readout of neuroaxonal damage and regeneration.\u003cbr\u003e\u003cbr\u003eStructurally, NEFM assembles into 10-nm filaments with a coiled-coil backbone and phosphorylated C-terminal sidearms that control filament spacing and cytoskeletal elasticity.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NEFM - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NEFM - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NEFM - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NEFM - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NEFM+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047310713197,"sku":"FY13056","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_8b7aeac1-72e6-433b-8cd1-961af7cdb22d.jpg?v=1772019423","url":"https:\/\/www.ebiohippo.com\/products\/nefm-antibody-nf-m-neurofilament-medium-bha17135958","provider":"BioHippo","version":"1.0","type":"link"}