{"product_id":"nek11-antibody-serine-threonine-protein-kinase-nek11-bha17136115","title":"NEK11 Antibody \/ Serine\/threonine-protein kinase Nek11","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNEK11 Antibody \/ Serine\/threonine-protein kinase Nek11 is a anti-NEK11 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NEK11\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eNEK11 antibody detects Serine\/threonine-protein kinase Nek11, a member of the NIMA-related kinase family that regulates DNA damage checkpoints and cell cycle progression. The UniProt recommended name is Serine\/threonine-protein kinase Nek11 (NEK11). This kinase functions downstream of ATM\/ATR signaling pathways and mediates G2\/M checkpoint arrest following genotoxic stress to allow DNA repair before mitosis.\u003cbr\u003e\u003cbr\u003eFunctionally, NEK11 antibody identifies a 645-amino-acid nuclear and cytoplasmic protein that becomes activated through phosphorylation in response to DNA damage. NEK11 phosphorylates and promotes the degradation of CDC25A phosphatase, inhibiting activation of CDK1-cyclin B and preventing premature entry into mitosis. By coupling checkpoint signaling to cell cycle control, NEK11 preserves genomic stability during replication stress and DNA repair. It also interacts with other checkpoint kinases such as CHK1 and PLK1, forming a regulatory network that ensures proper mitotic timing.\u003cbr\u003e\u003cbr\u003eThe NEK11 gene is located on chromosome 3q22.1 and is expressed in proliferative tissues including testis, thymus, and intestinal epithelium. Expression levels are highest in S and G2 phases, consistent with its role in cell cycle regulation. NEK11 activity integrates stress signals with checkpoint arrest and recovery mechanisms.\u003cbr\u003e\u003cbr\u003ePathologically, loss or mutation of NEK11 leads to genomic instability, defective DNA repair, and tumorigenesis. Downregulation is observed in colorectal, liver, and lung cancers, where impaired checkpoint function contributes to uncontrolled proliferation. Conversely, elevated NEK11 expression may sensitize tumor cells to genotoxic therapy by prolonging checkpoint arrest. Research using NEK11 antibody supports studies in DNA damage response, checkpoint regulation, and cancer biology.\u003cbr\u003e\u003cbr\u003eNEK11 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect checkpoint kinases. \u003cbr\u003e\u003cbr\u003eStructurally, Serine\/threonine-protein kinase Nek11 contains an N-terminal catalytic kinase domain with a conserved lysine essential for ATP binding and a C-terminal regulatory region mediating protein-protein interactions. Its activation loop undergoes autophosphorylation to enhance catalytic activity following DNA damage. This antibody enables analysis of NEK11's role in coordinating checkpoint signaling and maintaining genomic stability.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NEK11 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NEK11 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NEK11 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NEK11 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NEK11+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047318511981,"sku":"FY13213","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_0c6cafc5-bef7-40c8-88b8-6235f182a38b.jpg?v=1782237076","url":"https:\/\/www.ebiohippo.com\/products\/nek11-antibody-serine-threonine-protein-kinase-nek11-bha17136115","provider":"BioHippo","version":"1.0","type":"link"}