{"product_id":"nek5-antibody-nima-related-protein-kinase-5-bha17135294","title":"NEK5 Antibody \/ NimA-related protein kinase 5","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNEK5 Antibody \/ NimA-related protein kinase 5 is a anti-NEK5 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA with listed reactivity in Mouse, Rat. Reported localization: Nuclear, cytoplasmic.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NEK5\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC\/IF, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eThe NEK5 antibody targets Serine\/threonine-protein kinase Nek5, an enzyme encoded by the NEK5 gene and belonging to the NIMA-related kinase (NEK) family. These kinases regulate cell cycle progression, centrosome dynamics, and DNA damage responses. Serine\/threonine-protein kinase Nek5 is implicated in maintaining mitochondrial integrity, regulating apoptosis, and coordinating mitotic entry. The NEK5 antibody provides a valuable reagent for studying kinase signaling networks that ensure genomic stability and cellular homeostasis.\u003cbr\u003e\u003cbr\u003eSerine\/threonine-protein kinase Nek5 contains an N-terminal catalytic domain with conserved motifs for ATP binding and phosphorylation activity, and a C-terminal region responsible for subcellular localization and protein interactions. It localizes to centrosomes and mitochondria, suggesting dual functions in cell division and energy metabolism. The NEK5 antibody supports studies of these distinct localizations, allowing researchers to examine how Nek5 activity integrates cell-cycle control with mitochondrial health. Evidence indicates that Nek5 contributes to the initiation of centrosome disjunction, ensuring proper spindle formation during mitosis.\u003cbr\u003e\u003cbr\u003eBeyond its role in mitotic regulation, Serine\/threonine-protein kinase Nek5 has emerged as a mediator of mitochondrial dynamics and apoptosis resistance. It stabilizes mitochondrial membrane potential and reduces reactive oxygen species accumulation, linking Nek5 function to cellular stress adaptation. The NEK5 antibody helps reveal how mitochondrial Nek5 signaling intersects with pathways involving BCL2 family proteins and caspase activation. Studies have suggested that loss of Nek5 increases sensitivity to oxidative damage and promotes apoptotic cell death, highlighting its potential importance in tissue homeostasis and neuroprotection.\u003cbr\u003e\u003cbr\u003eAberrant Nek5 expression or activity may contribute to cancer development and progression. Overexpression has been observed in several tumor types, including breast and prostate cancers, where it may promote survival under genotoxic stress. The NEK5 antibody supports exploration of these oncogenic associations, enabling analysis of protein levels, phosphorylation status, and subcellular distribution in tumor models. Functional studies continue to investigate Nek5 as a possible target for kinase inhibition strategies aimed at restoring mitotic fidelity and limiting tumor growth.\u003cbr\u003e\u003cbr\u003eThe NEK5 antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry. It yields strong centrosomal and mitochondrial staining consistent with its dual localization.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NEK5 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NEK5 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NEK5 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NEK5 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NEK5+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047285875053,"sku":"FY12391","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_d9e1449d-1541-4828-a669-48b3ae4f228e.jpg?v=1782236985","url":"https:\/\/www.ebiohippo.com\/products\/nek5-antibody-nima-related-protein-kinase-5-bha17135294","provider":"BioHippo","version":"1.0","type":"link"}