{"product_id":"nelfa-antibody-negative-elongation-factor-a-bha17135729","title":"NELFA Antibody \/ Negative elongation factor A","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNELFA Antibody \/ Negative elongation factor A is a anti-NELFA Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NELFA\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eNELFA antibody detects Negative elongation factor A, a transcriptional regulator that controls RNA polymerase II pausing and transcription elongation. Encoded by the NELFA gene on chromosome 9q34.11, this protein is a core component of the negative elongation factor (NELF) complex, which also includes NELFB, NELFC\/D, and NELFE subunits. Together, these proteins transiently inhibit RNA polymerase II progression downstream of transcription start sites, enabling proper promoter-proximal pausing, RNA capping, and regulated gene expression.\u003cbr\u003e\u003cbr\u003eNELFA interacts with DSIF (DRB sensitivity-inducing factor) and phosphorylated RNA polymerase II to modulate transcriptional timing and elongation efficiency. Release from pausing is mediated by P-TEFb-dependent phosphorylation events that allow productive elongation. Through these mechanisms, NELFA coordinates transcriptional responses to developmental, environmental, and signaling cues.\u003cbr\u003e\u003cbr\u003eThe NELFA antibody is widely used in transcription, chromatin, and epigenetic regulation research to study RNA polymerase II pausing, elongation control, and transcriptional activation. Western blot analysis identifies a 66 kilodalton band corresponding to NELFA, while immunofluorescence reveals nuclear punctate staining associated with active transcription foci. This antibody supports studies investigating transcriptional regulation and pause-release mechanisms.\u003cbr\u003e\u003cbr\u003eAltered NELFA activity affects global gene expression programs, contributing to developmental disorders, cancer, and viral replication control. NELFA has also been linked to stress responses, where it modulates transcription restart following DNA damage or heat shock. The NELFA antibody provides a critical reagent for dissecting the molecular mechanisms of transcription elongation and RNA polymerase II regulation.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NELFA - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NELFA - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NELFA - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NELFA - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NELFA+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047303635309,"sku":"FY12827","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_756f6023-fd92-45a7-83f9-0800c9404786.jpg?v=1782237053","url":"https:\/\/www.ebiohippo.com\/products\/nelfa-antibody-negative-elongation-factor-a-bha17135729","provider":"BioHippo","version":"1.0","type":"link"}