{"product_id":"nesprin-3-antibody-syne3-bha17135949","title":"Nesprin 3 Antibody \/ SYNE3","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNesprin 3 Antibody \/ SYNE3 is a anti-SYNE3 Rabbit antibody Recombinant Rabbit Monoclonal clone 30S39 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SYNE3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 30S39, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eNesprin 3 antibody detects Spectrin repeat containing nuclear envelope protein 3, encoded by the SYNE3 gene. This protein, commonly known as Nesprin 3, is a component of the linker of nucleoskeleton and cytoskeleton (LINC) complex that spans the nuclear envelope. Unlike other Nesprin family members that link directly to actin, Nesprin 3 connects to intermediate filaments through interactions with plectin. Nesprin 3 antibody provides researchers with a key reagent to study nuclear positioning, mechanotransduction, and cytoskeletal organization.\u003cbr\u003e\u003cbr\u003eThe LINC complex plays a central role in transmitting mechanical forces between the cytoskeleton and the nucleus. By tethering the nuclear envelope to intermediate filament networks, Nesprin 3 contributes to nuclear anchorage during migration, differentiation, and mitosis. Research using Nesprin 3 antibody has shown that loss of this protein disrupts nuclear shape and mechanosensing, leading to altered transcriptional responses. These findings emphasize the importance of Nesprin 3 in structural integrity and gene regulation.\u003cbr\u003e\u003cbr\u003eDysfunction of Nesprin proteins, including Nesprin 3, has been linked to muscular dystrophies, cardiomyopathies, and neurological disorders. While most studies have focused on Nesprin 1 and Nesprin 2, evidence suggests that Nesprin 3 contributes to nuclear envelope stability and tissue homeostasis. Studies using Nesprin 3 antibody have associated its altered expression with cancer progression, where changes in nuclear deformability affect invasion and metastasis. These roles highlight the significance of SYNE3 in both health and disease.\u003cbr\u003e\u003cbr\u003eNesprin 3 antibody is widely applied in western blotting, immunofluorescence, and immunohistochemistry. Western blotting demonstrates isoform expression, immunofluorescence shows its localization at the nuclear envelope, and immunohistochemistry reveals its distribution in tissues such as muscle, epithelium, and nervous system. Functional studies have employed Nesprin 3 antibody in knockdown and rescue experiments, linking its absence to nuclear detachment and impaired motility.\u003cbr\u003e\u003cbr\u003eBy providing validated Nesprin 3 antibody reagents,\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SYNE3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SYNE3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SYNE3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SYNE3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SYNE3+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047310451053,"sku":"FY13047","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_ea351fc8-d8f2-485f-a57c-f7d778a0debb.jpg?v=1772019381","url":"https:\/\/www.ebiohippo.com\/products\/nesprin-3-antibody-syne3-bha17135949","provider":"BioHippo","version":"1.0","type":"link"}