{"product_id":"neto1-antibody-neuropilin-and-tolloid-like-protein-1-bha17135541","title":"NETO1 Antibody \/ Neuropilin and tolloid-like protein 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNETO1 Antibody \/ Neuropilin and tolloid-like protein 1 is a anti-NETO1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NETO1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eNETO1 antibody detects Neuropilin and tolloid-like protein 1, a neuronal transmembrane glycoprotein that modulates ionotropic glutamate receptor function and synaptic plasticity. NETO1 acts as an auxiliary subunit of kainate-type glutamate receptors (KARs), regulating receptor trafficking, channel gating, and synaptic localization. The NETO1 antibody is widely used in neurobiology and synaptic physiology to study excitatory neurotransmission and learning mechanisms.\u003cbr\u003e\u003cbr\u003eNETO1 is encoded by the NETO1 gene located on human chromosome 18q22.1. The protein is approximately 533 amino acids long and contains two extracellular CUB domains, a low-density lipoprotein receptor-like domain, a single transmembrane helix, and a short cytoplasmic tail. NETO1 is predominantly expressed in the hippocampus, cortex, and cerebellum, where it localizes to postsynaptic membranes and interacts with KAR subunits such as GluK2 and GluK5.\u003cbr\u003e\u003cbr\u003eThe NETO1 antibody detects a 63 kilodalton protein by western blot and shows punctate synaptic staining in neurons. NETO1 enhances the stability and functional diversity of KARs by modulating desensitization kinetics and surface expression. It contributes to long-term potentiation and synaptic scaling, processes underlying memory and learning. Mice lacking NETO1 display deficits in synaptic plasticity and spatial learning, confirming its critical role in excitatory neurotransmission.\u003cbr\u003e\u003cbr\u003eBeyond the brain, NETO1 may participate in developmental signaling and axonal growth. Dysregulation or mutation of NETO1 has been associated with autism spectrum disorder, schizophrenia, and cognitive impairment. By shaping receptor signaling and synaptic strength, NETO1 maintains proper excitatory balance within neural circuits.\u003cbr\u003e\u003cbr\u003eAs a neuron-specific regulatory subunit of kainate receptors, NETO1 is a key molecule for studying excitatory synaptic physiology and plasticity.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NETO1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NETO1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NETO1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NETO1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NETO1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047297081709,"sku":"FY12638","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_826beefe-b94d-44ac-874d-4ac169babd63.jpg?v=1782237035","url":"https:\/\/www.ebiohippo.com\/products\/neto1-antibody-neuropilin-and-tolloid-like-protein-1-bha17135541","provider":"BioHippo","version":"1.0","type":"link"}