{"product_id":"neu3-antibody-sialidase-3-bha17135874","title":"NEU3 Antibody \/ Sialidase 3","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNEU3 Antibody \/ Sialidase 3 is a anti-NEU3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NEU3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eNEU3 antibody detects Sialidase-3, a plasma membrane-associated enzyme that catalyzes the hydrolysis of sialic acid residues from gangliosides and glycoproteins. The UniProt recommended name is Sialidase-3 (NEU3). This enzyme is primarily localized at the plasma membrane and regulates signaling processes associated with cell growth, differentiation, and apoptosis through the modulation of membrane sialoglycoconjugates.\u003cbr\u003e\u003cbr\u003eFunctionally, NEU3 antibody identifies a 428-amino-acid enzyme that preferentially acts on gangliosides such as GM3, GD1a, and GT1b, catalyzing their desialylation. NEU3 activity alters the composition of lipid rafts, influencing membrane receptor function and downstream signaling. It modulates the activity of growth factor receptors, including EGFR and integrins, by affecting their sialic acid content and clustering at the plasma membrane. Through these mechanisms, NEU3 participates in the regulation of cell proliferation, adhesion, and migration.\u003cbr\u003e\u003cbr\u003eThe NEU3 gene is located on chromosome 11q13.5 and encodes a glycosidase that is anchored to the plasma membrane via hydrophobic domains. NEU3's enzymatic activity contributes to ganglioside catabolism and recycling, which are critical for maintaining lipid membrane homeostasis. Dysregulation of NEU3 expression has been linked to oncogenesis, as elevated levels promote tumor cell survival, motility, and resistance to apoptosis. Conversely, NEU3 deficiency impairs receptor-mediated signaling and disrupts ganglioside balance in neuronal and epithelial cells.\u003cbr\u003e\u003cbr\u003eIn cancer biology, NEU3 overexpression enhances epidermal growth factor receptor (EGFR) signaling by desialylating GM3, a natural EGFR inhibitor. This results in sustained receptor activation and downstream MAPK and PI3K\/AKT pathway stimulation. NEU3 also influences integrin-mediated adhesion and migration, contributing to metastasis. Beyond oncology, NEU3 is involved in immune modulation, neuronal signaling, and muscle differentiation. It also regulates autophagy by modulating membrane glycosphingolipid composition.\u003cbr\u003e\u003cbr\u003eNEU3 antibody is widely used in glycobiology, cancer research, and neurobiology to analyze sialidase function and membrane signaling. It supports applications including immunoblotting, immunocytochemistry, and enzyme localization studies. NEU3 expression profiling provides insight into tumor progression, inflammatory signaling, and metabolic disorders. The enzyme's surface localization makes it a potential therapeutic target for modulating receptor activity and glycosphingolipid metabolism.\u003cbr\u003e\u003cbr\u003eStructurally, NEU3 contains conserved catalytic motifs typical of sialidases and a hydrophobic transmembrane anchor that facilitates membrane association. It functions as a monomer and is regulated by pH and substrate availability.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NEU3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NEU3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NEU3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NEU3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NEU3+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047307239789,"sku":"FY12972","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_fdd32fd6-fb52-43de-9588-0dc691ca32f2.jpg?v=1782237061","url":"https:\/\/www.ebiohippo.com\/products\/neu3-antibody-sialidase-3-bha17135874","provider":"BioHippo","version":"1.0","type":"link"}