{"product_id":"neurod1-reporter-lentivirus-bhv19400218","title":"NeuroD1 Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eNeuroD1, also known as BETA2, is a basic helix-loop-helix transcription factor of the neural NeuroD family. It forms heterodimers with ubiquitous E-proteins and binds E-box elements to activate target genes including insulin, secretin, and pro-opiomelanocortin. NeuroD1 is expressed in neurons, the anterior pituitary, pancreatic islets, and enteroendocrine cells, where it directs neuronal differentiation, cerebral cortex development, and the maturation and expansion of insulin-producing pancreatic beta cells. At the secretin enhancer it cooperates synergistically with Sp1 and RREB1 to drive transcription. Because of its dual role in neural and endocrine programs, NeuroD1 is an important target in neuroscience, diabetes, and developmental biology research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe NeuroD1 Reporter Lentivirus measures NeuroD1 transcriptional activity in mammalian cells. It uses tandem repeats of NeuroD1 response elements taken from the secretin promoter enhancer, a context that reflects NeuroD1's synergistic interaction with Sp1 and RREB1, coupled to a minimal promoter driving a fluorescent or luminescent reporter. A constitutively expressed selection marker and optional secondary reporter support stable polyclonal reporter cell lines with readout by microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable lentiviral integration gives consistent reporter expression in dividing and post-mitotic cells, including primary and cryopreserved cultures, avoiding transient-transfection variability. Particles are purified by PEG precipitation and sucrose gradient centrifugation and transduce difficult-to-transfect cells, supporting research on NeuroD1 in neuronal development and pancreatic beta-cell biology.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the NeuroD1 transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251621126509,"sku":"LTV-0109-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53362031591789,"sku":"LTV-0109-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53362031624557,"sku":"LTV-0109-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53362031657325,"sku":"LTV-0109-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53362031690093,"sku":"LTV-0109-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53362031722861,"sku":"LTV-0109-6S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/NeuroD1-TAG-Puro-fig1.jpg?v=1776934215","url":"https:\/\/www.ebiohippo.com\/products\/neurod1-reporter-lentivirus-bhv19400218","provider":"BioHippo","version":"1.0","type":"link"}