{"product_id":"neuronatin-antibody-nnat-bha17135385","title":"Neuronatin Antibody \/ NNAT","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNeuronatin Antibody \/ NNAT is a anti-NNAT Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Immunohistochemistry (IHC), Immunofluorescence (IF), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NNAT\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e IHC, IF, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eNeuronatin antibody detects Neuronatin, a small endoplasmic reticulum-associated protein expressed primarily in the developing nervous system and endocrine tissues. Neuronatin plays an important role in neurogenesis, calcium signaling, and energy metabolism, and is regulated through genomic imprinting with expression derived exclusively from the paternal allele. The Neuronatin antibody is commonly used in neuroscience, developmental biology, and metabolic research to study neuronal differentiation, calcium regulation, and imprinting mechanisms associated with growth and metabolic control.\u003cbr\u003e\u003cbr\u003eNeuronatin is encoded by the NNAT gene on human chromosome 20q11.23. Two isoforms, NNAT-alpha and NNAT-beta, arise from alternative splicing and encode proteins of approximately 81 and 70 amino acids, respectively. Both isoforms are hydrophobic, integral membrane proteins localized to the endoplasmic reticulum. Structurally, Neuronatin shares sequence similarity with phospholamban and sarcolipin, suggesting a conserved role in modulating the sarco\/endoplasmic reticulum calcium ATPase (SERCA) pump. Through this interaction, Neuronatin influences calcium flux and storage, which are critical for neuronal excitability and hormone secretion.\u003cbr\u003e\u003cbr\u003eThe Neuronatin antibody reveals protein expression during embryonic and postnatal development in the brain, spinal cord, pituitary gland, and pancreas. Immunostaining shows localization within neuronal soma and processes, as well as in pancreatic beta cells, where Neuronatin contributes to insulin secretion. Western blot analysis typically detects a low molecular weight band corresponding to one or both isoforms. Functional studies demonstrate that Neuronatin enhances intracellular calcium release and supports the differentiation of neural progenitor cells by activating calcium-dependent transcriptional programs.\u003cbr\u003e\u003cbr\u003eBeyond its developmental functions, Neuronatin has been implicated in metabolic regulation and energy homeostasis. It modulates endoplasmic reticulum stress responses and may act as a mediator between nutrient sensing and neuronal metabolism. Dysregulation of NNAT expression has been associated with obesity, type 2 diabetes, and neurological disorders such as epilepsy and schizophrenia. Aberrant imprinting or epigenetic silencing of the paternal NNAT allele has been reported in human cancers, indicating a potential role in tumor suppression and differentiation control.\u003cbr\u003e\u003cbr\u003eNeuronatin expression persists in adult tissues with high metabolic demand, including hypothalamic nuclei, skeletal muscle, and endocrine pancreas. It functions as a calcium-dependent signal amplifier that adjusts energy utilization and cellular excitability. In neurons, NNAT influences synaptic development and may interact with signaling proteins that control axonal growth.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NNAT - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NNAT - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NNAT - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NNAT - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NNAT+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047289839981,"sku":"FY12482","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_d92b86ce-0705-4f30-bf40-e4b1b4fa30ef.jpg?v=1782237007","url":"https:\/\/www.ebiohippo.com\/products\/neuronatin-antibody-nnat-bha17135385","provider":"BioHippo","version":"1.0","type":"link"}