{"product_id":"nf-kappa-b-asc-pyroptosis-reporter-lentivirus-bhv19400285","title":"NFκB-ASC (Pyroptosis) Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003ePyroptosis is a pro-inflammatory form of programmed cell death triggered by inflammasome activation. When danger signals are sensed, the adaptor protein ASC polymerizes into a large cytoplasmic aggregate, the ASC speck, which serves as a platform for activation of caspase-1 (CASP1). Active caspase-1 cleaves the pore-forming protein gasdermin D (GSDMD) and proinflammatory cytokines, and the released GSDMD N-terminal fragment forms membrane pores that drive cell lysis and inflammation. The ASC speck is a hallmark of inflammasome assembly, and NF-κB signaling provides the priming step that upregulates inflammasome components, making ASC speck formation a valuable readout for studying pyroptosis and inflammation.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe NFκB-ASC (Pyroptosis) Reporter Lentivirus is an immunotherapy reporter system designed to monitor pyroptotic ASC speck formation in response to inflammasome activation. The system expresses a fluorescently tagged ASC fusion protein, available as ASC-GFP or ASC-RFP, so that ASC polymerization can be visualized directly as discrete specks marking inflammasome complex assembly; additional reporter configurations include fluorescent and secreted Gaussia luciferase outputs.\u003c\/p\u003e\n\u003cp\u003eStably expressed in suitable cells such as THP-1 monocytes, the reporter enables in vitro study of pyroptosis, a pro-inflammatory form of programmed cell death linked to caspase-1 and gasdermin D activity. Supplied as VSV-G-pseudotyped third-generation lentiviral particles purified by PEG precipitation and sucrose gradient centrifugation, the product transduces difficult-to-transfect cells, including primary and thawed cultures, supporting inflammasome and inflammation research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis 2-vial immunotherapy reporter system consists of a \u003cstrong\u003eVial 1 Receptor Lentivirus\u003c\/strong\u003e encoding human Pyroptosis Assay under a constitutive promoter with antibiotic selection, and a \u003cstrong\u003eVial 2 Reporter Lentivirus\u003c\/strong\u003e encoding tandem NFAT (or NF-κB) response elements driving a dual reporter (ASC-GFP Fusion Protein, ASC-RFP Fusion Protein, GFP, GFP-P2A-GLuc, GLuc, RFP, RFP-P2A-GLuc). Sequential transduction and selection generates a dual-stable effector cell line that responds quantitatively to receptor stimulation with a ratiometric fluorescent + bioluminescent readout.\u003c\/p\u003e\n\u003cp\u003eSecreted Gaussia luciferase (where included) accumulates in conditioned media, enabling kinetic sampling without cell lysis. The combined fluorescent and luminescent outputs allow parallel microscopy-based visualization and plate-reader luminometry from the same cell population — providing assay redundancy and flexibility for potency testing formats compliant with regulatory expectations for cell-based functional assays.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"ASC-GFP Fusion Protein \/ Blasticidin \/ 3.5x10^6","offer_id":53251623453037,"sku":"TRV-0037-1S","price":695.0,"currency_code":"USD","in_stock":true},{"title":"ASC-GFP Fusion Protein \/ Puromycin \/ 3.5x10^6","offer_id":53362028151149,"sku":"TRV-0037-2S","price":695.0,"currency_code":"USD","in_stock":true},{"title":"ASC-RFP Fusion Protein \/ Blasticidin \/ 3.5x10^6","offer_id":53362028183917,"sku":"TRV-0037-3S","price":695.0,"currency_code":"USD","in_stock":true},{"title":"ASC-RFP Fusion Protein \/ Puromycin \/ 3.5x10^6","offer_id":53362028216685,"sku":"TRV-0037-4S","price":695.0,"currency_code":"USD","in_stock":true},{"title":"ASC-GFP Fusion Protein \/ Puromycin \/ 5x10^6 cells","offer_id":53362028249453,"sku":"TRC-0037-1S","price":1495.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/pyroptosis-assay-thumbnail.jpg?v=1782157779","url":"https:\/\/www.ebiohippo.com\/products\/nf-kappa-b-asc-pyroptosis-reporter-lentivirus-bhv19400285","provider":"BioHippo","version":"1.0","type":"link"}