{"product_id":"nf-kappa-b-p65-reporter-lentivirus-bhv19400065","title":"NFκB (p65) Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eNF-κB is a family of transcription factors that coordinate immune responses, inflammation, cell survival, and proliferation. Active NF-κB complexes are dimers of Rel-family subunits; the p65 subunit, also called RelA, is a strong transcriptional activator. p65 most commonly partners with p50 in canonical signaling downstream of stimuli such as TNF-alpha, but it can also form p65\/p65 homodimers with distinct transcriptional properties. Selective monitoring of p65 homodimer activity is useful for dissecting non-canonical aspects of NF-κB signaling, including responses in tumor cells that are separate from classical inflammatory activation. Dysregulated NF-κB signaling contributes to chronic inflammation, autoimmunity, and cancer, making it an important focus of immunology research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe NF-κB (p65) Reporter Lentivirus is a transcription factor reporter system that provides a sensitive fluorescent or luminescent readout of p65 (RelA) homodimer activity in mammalian cells. The construct contains tandem repeats of the p65 DNA-binding element placed upstream of a minimal promoter driving the reporter gene, with an optimized upstream enhancer that maximizes signal-to-noise. Used alongside a pan-NF-κB reporter, it allows discrimination of p65\/p65 homodimer activity from p50\/p65 heterodimer activity. A constitutive drug selection marker (Blasticidin or Puromycin) enables generation of stable polyclonal reporter cell lines suitable for fluorescence microscopy, flow cytometry, or luminometry. Supplied as high-titer particles purified by PEG precipitation and sucrose gradient centrifugation, it transduces primary and difficult-to-transfect cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a d2GFP, EGFP, Firefly Luc, GFP, mCherry, RFP reporter gene under the control of tandem consensus response elements specific for the NFκB (p65 homodimer) transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251622797677,"sku":"LTV-0070-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53310899519853,"sku":"LTV-0070-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53310899552621,"sku":"LTV-0070-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53310899585389,"sku":"LTV-0070-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53310899618157,"sku":"LTV-0070-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53310899650925,"sku":"LTV-0070-6S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LTV-0070-NFkB-p65-Reporter-Figure1.jpg?v=1776934144","url":"https:\/\/www.ebiohippo.com\/products\/nf-kappa-b-p65-reporter-lentivirus-bhv19400065","provider":"BioHippo","version":"1.0","type":"link"}