{"product_id":"nf-kappa-b-reporter-lentivirus-bhv19400002","title":"NF-κB Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eNF-κB is a family of inducible transcription factors, including the subunits encoded by NFKB1 (p50) and RELA (p65), that control inflammation, innate and adaptive immunity, cell survival, and stress responses. In resting cells, NF-κB dimers are held inactive in the cytoplasm by IκB inhibitor proteins. Stimulation by cytokines such as TNF and IL-1, pathogen-associated molecules, or other stressors activates the IKK complex, which phosphorylates IκB and triggers its degradation. The freed NF-κB dimers translocate to the nucleus and bind κB DNA elements to drive transcription of inflammatory and pro-survival genes. Dysregulated NF-κB signaling underlies chronic inflammation, autoimmune disease, and cancer.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe NF-κB Reporter Lentivirus is a lentiviral reporter system that provides a sensitive fluorescent (GFP\/RFP) or luminescent readout of NF-κB pathway activity in human and mouse cells. The construct contains a minimal TATA promoter, tandem κB DNA-binding elements, and an optimized mini-enhancer that amplifies NF-κB-dependent reporter expression for strong signal-to-noise. An EF1a-driven selection marker (puromycin or blasticidin) enables easy generation of stable reporter cell lines. Supplied as high-titer, VSV-G-pseudotyped, serum-free particles purified by PEG precipitation and sucrose gradient centrifugation, the system transduces a broad range of cell types, including difficult-to-transfect primary and thawed cells, and supports readout by fluorescence microscopy, flow cytometry, or luminometry for inflammation, immune signaling, and pathway-modulator studies.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a BFP, Firefly Luc, GFP, GFP-P2A-FLuc, Luc, Renilla Luc, RFP, RFP-P2A-FLuc, SEAP, ZsGreen reporter gene under the control of tandem consensus response elements specific for the NFκB signaling pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, GFP (constitutively expressed), GFP-P2A-Puro (constitutively expressed), Puromycin, RFP (constitutively expressed), RFP-P2A-BSD (constitutively expressed)) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251623158125,"sku":"LTV-0002-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53251630956909,"sku":"LTV-0002-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53251630989677,"sku":"LTV-0002-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53251631022445,"sku":"LTV-0002-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53251631055213,"sku":"LTV-0002-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53251631087981,"sku":"LTV-0002-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"SEAP \/ Puromycin \/ 2x10^6","offer_id":53251631120749,"sku":"LTV-0002-7S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"SEAP \/ Blasticidin \/ 2x10^6","offer_id":53251631153517,"sku":"LTV-0002-8S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP-P2A-FLuc \/ Puromycin \/ 1x10^6","offer_id":53251631186285,"sku":"LTV-0002-9S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP-P2A-FLuc \/ Blasticidin \/ 1x10^6","offer_id":53251631219053,"sku":"LTV-0002-10S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP-P2A-FLuc \/ Puromycin \/ 1x10^6","offer_id":53251631251821,"sku":"LTV-0002-11S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP-P2A-FLuc \/ Blasticidin \/ 1x10^6","offer_id":53251631284589,"sku":"LTV-0002-12S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ RFP (constitutively expressed) \/ 2x10^6","offer_id":53251631317357,"sku":"LTV-0002-13S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ GFP (constitutively expressed) \/ 2x10^6","offer_id":53251631350125,"sku":"LTV-0002-14S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"BFP \/ Puromycin \/ 5x10^6","offer_id":53251631382893,"sku":"LTV-0002-15S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"BFP \/ Blasticidin \/ 5x10^6","offer_id":53251631415661,"sku":"LTV-0002-16S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Renilla Luc \/ Blasticidin \/ 2x10^6","offer_id":53251631448429,"sku":"LTV-0002-4SIC","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LTV-0002-NFkB-TAG-Puro-Fig.2-RAW264.7-stable-cell-line-1.jpg?v=1776934146","url":"https:\/\/www.ebiohippo.com\/products\/nf-kappa-b-reporter-lentivirus-bhv19400002","provider":"BioHippo","version":"1.0","type":"link"}