{"product_id":"nfat-gata-reporter-lentivirus-bhv19400245","title":"NFAT\/GATA Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eNFAT (nuclear factor of activated T cells) and GATA transcription factors cooperate to control cytokine gene expression in immune cells. The NFAT family (NFATc1-c4 and NFAT5) responds to calcium and calcineurin signaling downstream of antigen receptors and acts as an antigen-specific regulator of gene expression in T cells and myeloid cells. GATA3 is the master transcription factor of the Th2 lineage. At composite response elements, such as those in the IL-3 promoter, NFAT and GATA proteins bind together and synergistically activate Th2-associated cytokine genes including IL-4, IL-5, and IL-13. Reporters built on these composite elements provide a readout of NFAT\/GATA co-regulated transcription central to allergic and adaptive immune responses.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe NFAT\/GATA Reporter Lentivirus is a transcription-factor reporter system for detecting synergistic NFAT and GATA transcriptional activity in mammalian cells. The construct uses tandem repeats of composite NFAT and GATA DNA-binding elements derived from the human IL-3 promoter, coupled to a minimal promoter that drives a fluorescent or luminescent reporter, so the readout reflects co-regulated activation by both factors. A constitutively expressed selection marker and optional secondary reporter support stable polyclonal reporter cell lines.\u003c\/p\u003e\n\u003cp\u003eStable lentiviral integration provides consistent reporter expression in dividing and post-mitotic cells, including primary and cryopreserved cultures, avoiding transient-transfection variability. Particles are purified by PEG precipitation and sucrose gradient centrifugation and transduce difficult-to-transfect cells, supporting research on T-cell activation and Th2 cytokine regulation with readout by microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the NFAT + GATA Pathways transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"NFAT\/GATA-TAG-Puro \/ GFP \/ 5x10^6","offer_id":53251620929901,"sku":"LTV-0138-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Negative Control (NC-TAG-Puro) \/ GFP \/ 5x10^6","offer_id":53362089591149,"sku":"LTV-0138-1N","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Positive Control (PC-TAG-Puro) \/ GFP \/ 5x10^6","offer_id":53362089623917,"sku":"LTV-0138-1P","price":595.0,"currency_code":"USD","in_stock":true},{"title":"NFAT\/GATA-TAL-Puro \/ Firefly Luc \/ 2x10^6","offer_id":53362089656685,"sku":"LTV-0138-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Negative Control (NC-TAL-Puro) \/ Firefly Luc \/ 2x10^6","offer_id":53362089689453,"sku":"LTV-0138-3N","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Positive Control (PC-TAL-Puro) \/ Firefly Luc \/ 2x10^6","offer_id":53362089722221,"sku":"LTV-0138-3P","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Internal Control (RLuc-BSD) \/ Renilla Luc \/ 2x10^6","offer_id":53362089754989,"sku":"LTV-0138-3R","price":595.0,"currency_code":"USD","in_stock":true},{"title":"NFAT\/GATA-TAR-BSD \/ RFP \/ 5x10^6","offer_id":53362089787757,"sku":"LTV-0138-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"NFAT\/GATA-TAL-BSD \/ Firefly Luc \/ 2x10^6","offer_id":53362089820525,"sku":"LTV-0138-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"NFAT\/GATA-TAG-BSD \/ GFP \/ 5x10^6","offer_id":53362089853293,"sku":"LTV-0138-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"NFAT\/GATA-TAR-Puro \/ RFP \/ 5x10^6","offer_id":53362089886061,"sku":"LTV-0138-6S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/nfat-gata-2.jpg?v=1776934219","url":"https:\/\/www.ebiohippo.com\/products\/nfat-gata-reporter-lentivirus-bhv19400245","provider":"BioHippo","version":"1.0","type":"link"}