{"product_id":"nfat-luc-cmvh-stably-expressing-raw264-7-cell-line-bhc10923266","title":"NFAT‐luc‐CMVH Stably Expressing RAW264.7 Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThe NFAT-luc-CMVH Stable RAW264.7 Cell Line is a genetically engineered version of the RAW264.7 murine macrophage cell line, which stably expresses a luciferase reporter gene under the control of the Nuclear Factor of Activated T-cells (NFAT) promoter, combined with a cytomegalovirus (CMV) enhancer. This cell line is an essential tool for investigating the activation of NFAT, a key transcription factor involved in the regulation of immune response and in the development of T cells.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e NFAT‐luc‐CMVH Stably Expressing RAW264.7 Cell Line is supplied as an engineered cell line derived from Mouse ascites.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, dendritic-like\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. MEM α, No Nucleosides Medium (TM512) + 10% FBS(Regular*) + 2 mM L-Glutamine (G275) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEngineering \/ immortalization:\u003c\/strong\u003e Luciferase reporter expression.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in immunology, hematology, and signaling studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThe luciferase reporter allows for the real-time, non-destructive quantification of NFAT activation by emitting bioluminescence in response to calcium signaling, making it highly suitable for drug screening and cellular communication studies. This cell line is particularly useful in studies focused on understanding the dynamics of macrophage activation and the cellular mechanisms underlying inflammatory responses. Additionally, it can be used to assess the impact of pharmacological agents on NFAT pathway activation within macrophages, providing insights into potential therapeutic targets for diseases involving immune dysregulation.Complete list of cell lines from this panel:Cat. T6529 - NFAT‐luc‐CMVH Stably Expressing RAW264.7 Cell LineCat. T6530 - AP‐1‐luc‐CMVH Stably Expressing RAW264.7 Cell LineCat. T6531 - ARE‐luc‐CMVH Stably Expressing RAW264.7 Cell Line Donor\/background information provided for this product: Male, Balb\/c, Adult, Abelson murine leukemia virus-induced tumor. Expression information reported for the model: NFAT‐luc‐CMVH.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eEngineered cell lines are widely used for reporter-based readouts, perturbation studies, and assay optimization in reproducible culture systems.\u003c\/li\u003e\n\u003cli\u003eReporter or transgene-bearing models are often compared with matched parental or control cells to interpret signal changes in context.\u003c\/li\u003e\n\u003cli\u003eExpression trends are typically evaluated alongside passage number, selection pressure, and baseline growth behavior.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRoutine expansion and maintenance of a defined cell model for downstream in vitro experiments.\u003c\/li\u003e\n\u003cli\u003ePhenotype, signaling, or marker-expression studies performed under standardized culture conditions.\u003c\/li\u003e\n\u003cli\u003eCell-based assay development in which passage number, growth surface, and medium composition are tracked as experimental variables.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. MEM α, No Nucleosides Medium (TM512) + 10% FBS(Regular*) + 2 mM L-Glutamine (G275) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate\u003c\/li\u003e\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180552675693,"sku":"T6529","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/0GUSeZQuKdVK4GYcRW46SADCk8NXX3d0yQoGy4qy.png?v=1774957884","url":"https:\/\/www.ebiohippo.com\/products\/nfat-luc-cmvh-stably-expressing-raw264-7-cell-line-bhc10923266","provider":"BioHippo","version":"1.0","type":"link"}