{"product_id":"nfe2-antibody-nuclear-factor-erythroid-2-bha17135769","title":"NFE2 Antibody \/ Nuclear factor erythroid 2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNFE2 Antibody \/ Nuclear factor erythroid 2 is a anti-NFE2 Rabbit antibody Recombinant Rabbit Monoclonal clone 31N33 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP) with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NFE2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 31N33, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC, IF, IP\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eNFE2 antibody targets Nuclear factor erythroid 2, a transcription factor encoded by the NFE2 gene. NFE2 belongs to the basic leucine zipper family and is composed of a heterodimer of a large DNA-binding subunit (p45) and a small subunit from the Maf protein family. This transcription factor plays a central role in the regulation of erythroid and megakaryocytic cell differentiation. NFE2 antibody provides an essential reagent for studying gene expression programs controlling hematopoiesis, oxidative stress responses, and platelet production.\u003cbr\u003e\u003cbr\u003eThe p45 subunit of NFE2 binds to antioxidant response elements and regulates transcription of genes involved in heme biosynthesis, hemoglobin assembly, and detoxification of reactive oxygen species. In megakaryocytes, NFE2 is required for proplatelet formation and platelet release. Detection of this factor using NFE2 antibody helps identify regulatory pathways that maintain normal blood development and function.\u003cbr\u003e\u003cbr\u003eMutations or dysregulation of NFE2 are implicated in hematological disorders, including myeloproliferative neoplasms. Overexpression of NFE2 has been reported in polycythemia vera and essential thrombocythemia, linking the transcription factor to abnormal erythrocyte and platelet production. Research employing NFE2 antibody has revealed roles in leukemogenesis and abnormal megakaryopoiesis. Because of these connections, NFE2 is studied as a potential therapeutic target in blood cancers.\u003cbr\u003e\u003cbr\u003eNFE2 antibody can be applied in western blotting, immunohistochemistry, and electrophoretic mobility shift assays. Western blotting confirms protein expression in erythroid cell lines, while immunohistochemistry highlights tissue-specific nuclear localization. In gene regulation studies, chromatin immunoprecipitation with NFE2 antibody allows mapping of target promoters controlled by this transcription factor.\u003cbr\u003e\u003cbr\u003eNFE2 is also relevant in oxidative stress research. Its regulation of antioxidant genes links it to cellular defense mechanisms against free radicals and environmental toxins. Researchers using NFE2 antibody can explore how transcriptional control intersects with oxidative signaling and stress adaptation.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NFE2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NFE2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NFE2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NFE2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NFE2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047304618349,"sku":"FY12867","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_ec3ad383-6254-46cb-9a06-83013c4ff09f.jpg?v=1772019355","url":"https:\/\/www.ebiohippo.com\/products\/nfe2-antibody-nuclear-factor-erythroid-2-bha17135769","provider":"BioHippo","version":"1.0","type":"link"}