{"product_id":"nfe2-reporter-lentivirus-bhv19400064","title":"NFE2 Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eNFE2 (nuclear factor, erythroid 2, 45 kDa subunit) is a basic leucine zipper transcription factor essential for hematopoietic differentiation. It forms heterodimers with small MAF proteins, such as MAF-G, that bind NFE2 DNA elements and regulate gene expression. NFE2 is required for normal erythroid maturation and is a critical regulator of megakaryocyte development and platelet production. Loss of NFE2 function disrupts platelet biogenesis, while dysregulated NFE2 activity has been linked to myeloproliferative disorders. Through its control of erythroid and megakaryocytic gene programs, NFE2-dependent transcription is an informative readout in hematopoiesis and blood cell biology research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe NFE2 Reporter Lentivirus is a transcription-factor reporter system that provides a sensitive fluorescent or luminescent readout of human or mouse NFE2 activity. The construct uses consensus NFE2 DNA-binding elements derived from the PBGD promoter, coupled to a minimal promoter, to drive a reporter gene, while a constitutively expressed selection marker supports stable cell line generation. Reporter options include fluorescent proteins and luciferase, with readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eThe system is suited to studying NFE2 activity in erythroid and megakaryocytic differentiation, platelet biology, and related hematologic processes. Particles are purified by PEG precipitation and sucrose gradient centrifugation, enabling effective transduction of difficult-to-transfect primary and thawed cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a d2GFP, EGFP, Firefly Luc, GFP, mCherry, RFP reporter gene under the control of tandem consensus response elements specific for the NFE2 transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251620962669,"sku":"LTV-0069-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53310903091565,"sku":"LTV-0069-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53310903124333,"sku":"LTV-0069-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53310903157101,"sku":"LTV-0069-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53310903189869,"sku":"LTV-0069-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53310903222637,"sku":"LTV-0069-6S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/NFE2-reporter-panel.jpg?v=1776934145","url":"https:\/\/www.ebiohippo.com\/products\/nfe2-reporter-lentivirus-bhv19400064","provider":"BioHippo","version":"1.0","type":"link"}