{"product_id":"nfia-antibody-nuclear-factor-1-a-type-bha17135356","title":"NFIA Antibody \/ Nuclear factor 1 A-type","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNFIA Antibody \/ Nuclear factor 1 A-type is a anti-NFIA Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NFIA\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eNFIA antibody detects Nuclear factor 1 A-type, a member of the NFI transcription factor family involved in gene regulation during development, differentiation, and glial cell maturation. NFIA functions as both an activator and repressor of transcription and binds to the palindromic NFI recognition site present in numerous promoters. It plays critical roles in the development of the central nervous system, particularly in astrocyte differentiation, corpus callosum formation, and regulation of glial-specific gene expression. The NFIA antibody is a widely used tool for exploring transcriptional mechanisms that control neural development and glial identity.\u003cbr\u003e\u003cbr\u003eNFIA is encoded by the NFIA gene located on human chromosome 1p31.3. The protein contains a conserved N-terminal DNA-binding domain responsible for dimerization and sequence-specific binding, along with a C-terminal transactivation\/repression region that interacts with other transcriptional regulators. NFIA forms homo- and heterodimers with other NFI family members (NFIB, NFIC, NFIX), allowing combinatorial control of target genes. Studies in model organisms have shown that loss of NFIA disrupts gliogenesis, leading to abnormal brain structure and impaired glial differentiation.\u003cbr\u003e\u003cbr\u003eExpression profiling reveals that NFIA is abundant in glial progenitor cells and astrocytes, while its expression declines as neurons mature. Western blot analysis using NFIA antibody typically identifies a band near 54-60 kDa (unphosphorylated form), corresponding to the full-length transcription factor. Immunohistochemistry reveals strong nuclear localization in developing brain regions, spinal cord, and other tissues undergoing differentiation. NFIA cooperates with SOX9, STAT3, and other transcriptional factors to regulate astrocytic gene programs, such as those encoding GFAP and S100B.\u003cbr\u003e\u003cbr\u003eIn addition to its role in neurodevelopment, NFIA participates in the regulation of kidney morphogenesis, adipogenesis, and hematopoietic stem cell maintenance. Dysregulation or mutation of NFIA has been linked to developmental delay, macrocephaly, and agenesis of the corpus callosum in human syndromic cases.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NFIA - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NFIA - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NFIA - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NFIA - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NFIA+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047286595949,"sku":"FY12453","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_b1d87de2-2de4-45f0-9d07-5fc23195958a.jpg?v=1782236999","url":"https:\/\/www.ebiohippo.com\/products\/nfia-antibody-nuclear-factor-1-a-type-bha17135356","provider":"BioHippo","version":"1.0","type":"link"}