{"product_id":"nfkbib-antibody-ikb-beta-bha17136106","title":"NFKBIB Antibody \/ IkB Beta","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNFKBIB Antibody \/ IkB Beta is a anti-NFKBIB Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NFKBIB\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eNFKBIB antibody detects Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor beta, commonly known as IkB beta, a cytoplasmic protein that regulates NF-kappaB signaling by sequestering transcription factors in an inactive state. The UniProt recommended name is NF-kappaB inhibitor beta (NFKBIB). This regulatory protein acts as part of the I-kappaB family, which maintains NF-kappaB dimers in the cytoplasm under resting conditions, preventing their nuclear translocation and transcriptional activation of inflammatory genes.\u003cbr\u003e\u003cbr\u003eFunctionally, NFKBIB antibody identifies a 359-amino-acid protein containing six ankyrin repeats that mediate high-affinity binding to NF-kappaB subunits such as RELA (p65) and NFKB1 (p50). Upon cellular stimulation by cytokines, stress, or microbial components, NFKBIB undergoes phosphorylation by IKK kinases, followed by ubiquitination and proteasomal degradation. This releases NF-kappaB dimers, allowing them to enter the nucleus and activate transcription of immune, survival, and stress-response genes. The subsequent resynthesis of IkB beta provides negative feedback to terminate NF-kappaB signaling, restoring homeostasis.\u003cbr\u003e\u003cbr\u003eThe NFKBIB gene is located on chromosome 19q13.2 and is expressed broadly in immune, epithelial, and neuronal tissues. Its expression is dynamically regulated by inflammatory stimuli, making it a critical component of feedback loops that control cytokine production and immune tolerance.\u003cbr\u003e\u003cbr\u003ePathologically, dysregulation of NFKBIB contributes to chronic inflammation, autoimmune disorders, and cancer. Mutations or reduced degradation can suppress immune activation, while excessive turnover enhances NF-kappaB signaling and proinflammatory gene expression. Research using NFKBIB antibody supports studies in transcriptional regulation, inflammatory signaling, and tumor biology.\u003cbr\u003e\u003cbr\u003eNFKBIB antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect I-kappaB family proteins and NF-kappaB signaling regulators. \u003cbr\u003e\u003cbr\u003eStructurally, NF-kappaB inhibitor beta contains a signal response domain near the N-terminus for IKK-mediated phosphorylation and a C-terminal ankyrin repeat domain that anchors NF-kappaB subunits. This antibody enables the study of IkB beta's role in feedback regulation of NF-kappaB activity and immune homeostasis.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NFKBIB - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NFKBIB - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NFKBIB - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NFKBIB - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NFKBIB+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047317987693,"sku":"FY13204","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_f911a48c-1f1e-427b-b83a-65913c1404f2.jpg?v=1782237067","url":"https:\/\/www.ebiohippo.com\/products\/nfkbib-antibody-ikb-beta-bha17136106","provider":"BioHippo","version":"1.0","type":"link"}