{"product_id":"nfs1-antibody-cysteine-desulfurase-bha17136275","title":"NFS1 Antibody \/ Cysteine desulfurase","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNFS1 Antibody \/ Cysteine desulfurase is a anti-NFS1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunoprecipitation (IP), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NFS1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IP, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eNFS1 antibody detects Cysteine desulfurase, a mitochondrial enzyme encoded by the NFS1 gene on chromosome 20q11.22. NFS1 is essential for the biosynthesis of iron-sulfur (Fe-S) clusters, which serve as cofactors for a wide range of enzymes involved in electron transport, metabolic catalysis, and DNA repair. NFS1 catalyzes the conversion of L-cysteine to alanine, providing sulfur atoms for Fe-S cluster assembly within the mitochondrial matrix. This reaction represents the first committed step of Fe-S cluster formation, fundamental to mitochondrial respiration and cellular energy metabolism.\u003cbr\u003e\u003cbr\u003eStructurally, NFS1 is a pyridoxal phosphate (PLP)-dependent enzyme that functions as a homodimer. It forms a core part of the Fe-S cluster assembly complex with ISCU (scaffold protein), ISD11, ACP (acyl carrier protein), and FXN (Frataxin). NFS1 donates sulfur from cysteine via a persulfide intermediate, which is transferred to ISCU for subsequent cluster assembly. The protein belongs to the cysteine desulfurase family, conserved across prokaryotes and eukaryotes. Co-localization studies show NFS1 residing in the mitochondrial matrix, where it coexists with other Fe-S biosynthesis factors such as ISCU and FXN.\u003cbr\u003e\u003cbr\u003eFunctionally, NFS1 supports the biogenesis of Fe-S cluster-dependent enzymes including aconitase, succinate dehydrogenase, and DNA polymerase complexes. It plays a key role in oxidative phosphorylation, the tricarboxylic acid (TCA) cycle, and cellular redox homeostasis. In addition to mitochondrial roles, NFS1 also provides sulfur for cytosolic Fe-S protein assembly via the iron-sulfur cluster export machinery. Known interaction partners include ISD11, FXN, and NUBP2, which together ensure the stability and efficiency of sulfur transfer and cluster formation.\u003cbr\u003e\u003cbr\u003eDysregulation or mutation of NFS1 disrupts Fe-S cluster biogenesis and results in severe mitochondrial dysfunction. Deficiency causes combined oxidative phosphorylation deficiency 20 (COXPD20), characterized by lactic acidosis, developmental delay, and myopathy. In cancer, altered NFS1 expression influences metabolic reprogramming and redox balance. Pathway associations include iron-sulfur cluster biosynthesis, oxidative phosphorylation, and mitochondrial sulfur metabolism. Expression of NFS1 is particularly high in metabolically active tissues such as heart, liver, and skeletal muscle.\u003cbr\u003e\u003cbr\u003eThe NFS1 antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NFS1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NFS1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NFS1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NFS1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NFS1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047324574061,"sku":"FY13373","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_09fcb32e-0089-49d2-b4f1-a98a57e833f3.jpg?v=1782237112","url":"https:\/\/www.ebiohippo.com\/products\/nfs1-antibody-cysteine-desulfurase-bha17136275","provider":"BioHippo","version":"1.0","type":"link"}