{"product_id":"nid1-antibody-nidogen-1-entactin-bha17135234","title":"NID1 Antibody \/ Nidogen 1 \/ Entactin","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNID1 Antibody \/ Nidogen 1 \/ Entactin is a anti-NID1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NID1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eNID1 antibody is used to study Nidogen 1, also known as Entactin, a key basement membrane linker that bridges laminin networks with collagen IV scaffolds to establish tissue architecture and mechanical stability. As a secreted glycoprotein with multiple binding modules, Nidogen 1 connects laminin gamma chains to collagen IV and perlecan, organizing a resilient sheet that supports epithelial polarity, filtration barriers, and neuromuscular junctions. Through these interactions, the protein shapes morphogenesis, angiogenesis, and repair, and it helps buffer tissues against mechanical stress.\u003cbr\u003e\u003cbr\u003eNidogen 1 contains globular domains separated by flexible segments that adapt to different extracellular partners. Its localization along the basement membrane enables crosstalk between matrix and cells, influencing receptor signaling through integrins and dystroglycan. Depletion in animal models disrupts organogenesis and compromises barrier function in kidney and vasculature, highlighting the protein's structural and signaling roles. Because matrix composition tunes growth factor availability, Nidogen 1 also contributes to gradient formation and ligand presentation within tissue microenvironments.\u003cbr\u003e\u003cbr\u003eResearchers use NID1 antibody \/ Entactin in immunohistochemistry to delineate basement membranes in skin, kidney, lung, and tumor samples, providing clear borders for morphometric analyses and pathology scoring. In immunoblotting, the reagent tracks expression during development or matrix remodeling, while immunofluorescence co-localization with laminin and collagen IV reveals network integrity and continuity. In vitro, combining the antibody with matrix assembly assays clarifies how Nidogen 1 scaffolds influence cell adhesion, migration, and polarity under defined conditions.\u003cbr\u003e\u003cbr\u003eThe NID1 antibody \/ Entactin from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NID1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NID1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NID1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NID1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NID1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047287284077,"sku":"FY12331","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_128c4e21-076f-45c8-99d5-4003133dff31.jpg?v=1782237003","url":"https:\/\/www.ebiohippo.com\/products\/nid1-antibody-nidogen-1-entactin-bha17135234","provider":"BioHippo","version":"1.0","type":"link"}