{"product_id":"nip7-antibody-nucleolar-pre-rrna-processing-protein-nip7-bha17135384","title":"NIP7 Antibody \/ Nucleolar pre-rRNA processing protein NIP7","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNIP7 Antibody \/ Nucleolar pre-rRNA processing protein NIP7 is a anti-NIP7 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NIP7\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eNIP7 antibody detects Nucleolar pre-rRNA processing protein NIP7, a highly conserved factor involved in the biogenesis of the small ribosomal subunit. NIP7 participates in pre-rRNA processing and ribosome assembly within the nucleolus, contributing to the accurate formation of mature 18S rRNA. The NIP7 antibody is commonly used in molecular and cellular biology research to examine ribosome biogenesis, nucleolar function, and RNA processing mechanisms that sustain cell growth and proliferation.\u003cbr\u003e\u003cbr\u003eNIP7 is encoded by the NIP7 gene located on human chromosome 18q12.1. The protein is approximately 20 kilodaltons in size and localized predominantly in the nucleolus, where it associates with pre-ribosomal particles. Structurally, NIP7 contains an Sm-like fold and RNA-binding motifs enabling it to interact with ribosomal RNA precursors and small nucleolar RNAs. It forms complexes with NOP8 and RRP12, essential for the processing of 20S pre-rRNA and export of pre-40S subunits to the cytoplasm. These interactions make NIP7 a critical factor in maintaining ribosome synthesis efficiency.\u003cbr\u003e\u003cbr\u003eThe NIP7 antibody is valuable for detecting nucleolar proteins by western blot or immunocytochemistry. Western blot typically shows a distinct band at approximately 25 kilodaltons, while immunofluorescence highlights punctate nucleolar localization that co-stains with fibrillarin or nucleolin. Depletion of NIP7 disrupts 18S rRNA maturation, leading to defective ribosome assembly and impaired cell growth. Beyond ribosome synthesis, NIP7 may participate in broader RNA processing pathways, linking ribosome production to cell cycle progression and metabolic control.\u003cbr\u003e\u003cbr\u003eNIP7 is evolutionarily conserved from yeast to humans, emphasizing its fundamental role in RNA biology. Altered expression has been noted in certain cancers and stress conditions that perturb ribosome biogenesis.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NIP7 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NIP7 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NIP7 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NIP7 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NIP7+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047289774445,"sku":"FY12481","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_1d118cde-80c3-4bdc-bd41-e1f10acf236a.jpg?v=1782237014","url":"https:\/\/www.ebiohippo.com\/products\/nip7-antibody-nucleolar-pre-rrna-processing-protein-nip7-bha17135384","provider":"BioHippo","version":"1.0","type":"link"}