{"product_id":"nipah-virus-detection-kit-fluorescent-pcr-method-bht15200050","title":"Nipah Virus Detection Kit (Fluorescent PCR Method)","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-license-box{background:#f5f5f5;border:1px solid #ddd;border-radius:4px;padding:12px 16px;font-size:14px;color:#444}.bhp-desc .bhp-regulatory-row{display:flex;gap:16px;flex-wrap:wrap;margin:6px 0 10px}.bhp-desc .bhp-reg-item{background:#f0f6ff;border:1px solid #ccd9f0;border-radius:4px;padding:6px 12px;font-size:14px}.bhp-desc .bhp-reg-item strong{color:#003366;display:block;font-size:12px;text-transform:uppercase;letter-spacing:.5px}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}.bhp-desc .bhp-comp-table th{width:auto}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003ePathogen Detection Kits (qPCR)\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eNipah virus (NiV) is a zoonotic RNA virus belonging to the genus Henipavirus of the family Paramyxoviridae, together with Hendra virus. It was first identified in 1998 in Nipah village, Malaysia. Nipah virus infection can cause a range of clinical manifestations, including asymptomatic infection, acute respiratory disease, and fatal encephalitis, with an estimated mortality rate of 40–75%. The virus poses a significant threat to both human and animal health. Therefore, the establishment of rapid and accurate detection methods is of great importance for Nipah virus research.\u003c\/p\u003e\n\u003cp\u003eThis kit uses a fluorescent PCR (qPCR) method to detect the presence of Nipah virus in samples. The kit contains primers specifically designed for the conserved region of the NiV P protein, HotStarTaq DNA Polymerase, and an optimized qPCR buffer system, which together enhance amplification efficiency and enable effective amplification of low-concentration templates. Each 20 µL reaction is cycled at 95°C for 30 sec, then 35–40 cycles of 95°C for 5 sec and 60°C for 30 sec, followed by melting curve analysis.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eMethod\u003c\/th\u003e\n\u003ctd\u003eFluorescent PCR (qPCR)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget\u003c\/th\u003e\n\u003ctd\u003eConserved region of the NiV P protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePolymerase\u003c\/th\u003e\n\u003ctd\u003eHotStarTaq DNA Polymerase\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTemplate\u003c\/th\u003e\n\u003ctd\u003eDNA; no reverse transcription step\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eReaction volume\u003c\/th\u003e\n\u003ctd\u003e20 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eROX\u003c\/th\u003e\n\u003ctd\u003eROX Reference Dye supplied; volume varies by instrument\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eControls included\u003c\/th\u003e\n\u003ctd\u003eNiV Positive Control\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eReactions per kit\u003c\/th\u003e\n\u003ctd\u003e50 rxn or 100 rxn\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table bhp-comp-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003e50 rxn\u003c\/th\u003e\n\u003cth\u003e100 rxn\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e2× NiV qPCR Mix\u003c\/td\u003e\n\u003ctd\u003e500 µL\u003c\/td\u003e\n\u003ctd\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePrimer Mix\u003c\/td\u003e\n\u003ctd\u003e50 µL\u003c\/td\u003e\n\u003ctd\u003e100 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eNiV Positive Control\u003c\/td\u003e\n\u003ctd\u003e250 µL\u003c\/td\u003e\n\u003ctd\u003e500 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eROX Reference Dye\u003c\/td\u003e\n\u003ctd\u003e750 µL\u003c\/td\u003e\n\u003ctd\u003e1.5 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRNase-free ddH₂O\u003c\/td\u003e\n\u003ctd\u003e750 µL\u003c\/td\u003e\n\u003ctd\u003e1.5 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eResearch-use detection of Nipah virus sequences in experimental samples\u003c\/li\u003e\n\u003cli\u003eNipah virus research\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eKey Features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eReady-to-use master mix; the user supplies the DNA template.\u003c\/li\u003e\n\u003cli\u003ePrimers designed against a conserved region of the NiV P protein for high specificity; contact BioHippo for cross-reactivity data.\u003c\/li\u003e\n\u003cli\u003eNiV Positive Control included.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eStorage \u0026amp; Handling\u003c\/h2\u003e\n\u003cp\u003eStored at -20°C and protected from light, with a shelf life of one year. Shipping: Ice packs or dry ice.\u003c\/p\u003e\n\u003ch2\u003eUsage Notes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eWear a lab coat and disposable gloves during the experimental procedures.\u003c\/li\u003e\n\u003cli\u003eTransport the kit with ice packs or dry ice.\u003c\/li\u003e\n\u003cli\u003eAvoid strong light exposure.\u003c\/li\u003e\n\u003cli\u003eMix gently before use.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Regulatory\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp\u003eFor research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.\u003c\/p\u003e\u003c\/div\u003e\n\u003cdiv class=\"bhp-regulatory-row\"\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eGrade\u003c\/strong\u003eResearch Use Only\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eManufacturer\u003c\/strong\u003eELK Biotechnology\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eCatalog No.\u003c\/strong\u003eEQ041\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eBioHippo SKU\u003c\/strong\u003eBHT15200050\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ELK Biotechnology","offers":[{"title":"50 rxn","offer_id":53965513228653,"sku":"EQ041-50RXN","price":1040.0,"currency_code":"USD","in_stock":true},{"title":"100 rxn","offer_id":53965515030893,"sku":"EQ041-100RXN","price":1950.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/EQ041-a-1.jpg?v=1789419320","url":"https:\/\/www.ebiohippo.com\/products\/nipah-virus-detection-kit-fluorescent-pcr-method-bht15200050","provider":"BioHippo","version":"1.0","type":"link"}