{"product_id":"nkr1-antibody-neurokinin-1-receptor-tacr1-bha17135741","title":"NKR1 Antibody \/ Neurokinin 1 Receptor \/ TACR1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNKR1 Antibody \/ Neurokinin 1 Receptor \/ TACR1 is a anti-NKR1 Rabbit antibody Recombinant Rabbit Monoclonal clone AFBH-20 supplied in Liquid format. Recommended for workflows such as Immunoprecipitation (IP), Immunohistochemistry (IHC), Western blot (WB) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm, cell membrane.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NKR1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone AFBH-20, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e IP, IHC, WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eNKR1 antibody detects Neurokinin 1 receptor, a G protein-coupled receptor (GPCR) encoded by the TACR1 gene on chromosome 2p12 that mediates the biological effects of the neuropeptide Substance P. This receptor plays a central role in neurotransmission, pain perception, inflammation, and stress responses. Neurokinin 1 receptor belongs to the tachykinin receptor family, characterized by seven transmembrane helices, extracellular ligand-binding loops, and cytoplasmic regions that couple to G proteins for intracellular signaling.\u003cbr\u003e\u003cbr\u003eBinding of Substance P to the Neurokinin 1 receptor triggers activation of phospholipase C (PLC), leading to inositol trisphosphate (IP3) production, calcium release, and activation of protein kinase C (PKC). Downstream pathways include MAPK, ERK, and NF-kB signaling cascades that regulate neuronal excitability, cytokine production, and vascular permeability. This receptor is broadly expressed in the central nervous system-particularly in pain-processing regions such as the dorsal horn of the spinal cord-and in peripheral tissues including smooth muscle, endothelial cells, and immune cells.\u003cbr\u003e\u003cbr\u003eThe NKR1 antibody is widely used in neuroscience, pharmacology, and inflammation research to study Substance P signaling, pain transmission, and GPCR-mediated pathways. Western blot analysis detects a 46 kilodalton band corresponding to the receptor, while immunohistochemistry reveals strong membrane and cytoplasmic staining in neurons and smooth muscle cells. This antibody supports investigations into tachykinin signaling and receptor modulation across different tissues.\u003cbr\u003e\u003cbr\u003ePharmacologically, Neurokinin 1 receptor antagonists such as aprepitant and fosaprepitant are used clinically to treat nausea, vomiting, and depression, underscoring the receptor's therapeutic relevance. Dysregulation of TACR1 signaling contributes to chronic pain, anxiety, inflammatory bowel disease, and certain cancers, where Substance P promotes cell proliferation and angiogenesis. The NKR1 antibody provides a valuable tool for characterizing receptor expression, ligand binding, and intracellular signaling dynamics under physiological and pathological conditions.\u003cbr\u003e\u003cbr\u003eAt the molecular level, Neurokinin 1 receptor undergoes desensitization and internalization following ligand binding, a process mediated by beta-arrestins and receptor phosphorylation. It can recycle back to the plasma membrane or undergo degradation depending on signal intensity. The NKR1 antibody facilitates the study of receptor trafficking, desensitization, and GPCR signaling integration.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NKR1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NKR1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NKR1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NKR1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NKR1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047302979949,"sku":"FY12839","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_488e7f20-47a9-4201-a335-497ee8b213f6.jpg?v=1782237048","url":"https:\/\/www.ebiohippo.com\/products\/nkr1-antibody-neurokinin-1-receptor-tacr1-bha17135741","provider":"BioHippo","version":"1.0","type":"link"}