{"product_id":"nle1-antibody-notchless-protein-homolog-1-bha17135319","title":"NLE1 Antibody \/ Notchless protein homolog 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNLE1 Antibody \/ Notchless protein homolog 1 is a anti-NLE1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NLE1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eThe NLE1 antibody targets Notchless protein homolog 1, a nucleolar assembly factor encoded by the NLE1 gene. Notchless protein homolog 1 is required for ribosome biogenesis, pre-60S subunit maturation, and proper cell-cycle progression. Originally identified for its genetic interaction with the Notch pathway, NLE1 participates in both ribosomal RNA processing and developmental signaling. The NLE1 antibody enables researchers to study nucleolar organization, protein-synthesis control, and cross-talk between growth and differentiation pathways.\u003cbr\u003e\u003cbr\u003eNotchless protein homolog 1 contains WD-repeat motifs that facilitate interactions with nucleolar proteins involved in ribosome assembly, including NOP7 and RPF2. It promotes 60S subunit maturation by assisting in rRNA folding and ribosomal protein incorporation. The NLE1 antibody supports localization studies showing strong nucleolar enrichment, consistent with its essential role in ribosome production. Depletion of NLE1 results in defective ribosome biogenesis, p53 activation, and cell-cycle arrest.\u003cbr\u003e\u003cbr\u003eDuring embryonic development, Notchless protein homolog 1 influences cell-fate determination through modulation of Notch receptor signaling. Loss of NLE1 disrupts somite segmentation and neurogenesis in animal models. The NLE1 antibody allows analysis of these regulatory interactions, helping delineate how nucleolar activity intersects with developmental gene networks. Its dual role in ribosome formation and signaling regulation underscores the integration of biosynthetic and morphogenetic processes.\u003cbr\u003e\u003cbr\u003eIn cancer research, altered ribosome biogenesis and nucleolar stress contribute to tumorigenesis. Notchless protein homolog 1 expression correlates with proliferative capacity, making it a useful marker for studies of nucleolar dynamics in malignant tissues. The NLE1 antibody supports evaluation of nucleolar size, rRNA synthesis rate, and ribosomal assembly integrity in cancer and stress-response models.\u003cbr\u003e\u003cbr\u003eThe NLE1 antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, showing prominent nucleolar staining.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NLE1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NLE1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NLE1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NLE1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NLE1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047285940589,"sku":"FY12416","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_e8c02bbd-c1be-4073-9b0e-7c4e8850fed1.jpg?v=1782237004","url":"https:\/\/www.ebiohippo.com\/products\/nle1-antibody-notchless-protein-homolog-1-bha17135319","provider":"BioHippo","version":"1.0","type":"link"}