{"product_id":"nmi-antibody-n-myc-and-stat-interactor-bha17135498","title":"NMI Antibody \/ N myc and STAT interactor","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNMI Antibody \/ N myc and STAT interactor is a anti-NMI Rabbit antibody Recombinant Rabbit Monoclonal clone 30N95 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP) with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NMI\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 30N95, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC, IF, IP\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eNMI antibody detects N myc and STAT interactor, a coactivator protein encoded by the NMI gene. NMI interacts with transcription factors such as STATs and c-Myc, enhancing their transcriptional activity. It plays roles in cytokine signaling, oncogene regulation, and immune responses. By linking signal transduction to gene expression, NMI contributes to cellular responses to external cues.\u003cbr\u003e\u003cbr\u003eNMI antibody is widely used in immunology, oncology, and signal transduction research. NMI expression is induced by interferons and other cytokines, where it modulates STAT mediated transcription. It also interacts with c-Myc and N-Myc, affecting oncogenic transcriptional programs. By detecting NMI, researchers can explore how transcriptional coactivators regulate immune signaling and cancer pathways.\u003cbr\u003e\u003cbr\u003e In western blot assays, NMI antibody identifies protein bands of expected size in cytokine stimulated cells. Immunohistochemistry reveals expression in immune tissues, while immunofluorescence highlights nuclear and cytoplasmic distribution depending on activation state. These applications provide robust tools for analyzing NMI activity in signaling networks.\u003cbr\u003e\u003cbr\u003eNMI has been implicated in tumorigenesis. Its expression is elevated in breast and cervical cancers, where it interacts with oncogenes and alters signaling outcomes. Conversely, NMI may also play tumor suppressive roles in certain contexts, reflecting its complex regulation. By applying NMI antibody, scientists can dissect the dual roles of this coactivator in cancer biology.\u003cbr\u003e\u003cbr\u003eNMI also regulates antiviral responses and inflammation by modulating STAT signaling downstream of interferons. This positions NMI as a bridge between innate immunity and transcriptional responses. Its interactions with multiple transcription factors highlight its versatility in shaping gene expression outcomes.\u003cbr\u003e\u003cbr\u003eNMI antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NMI - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NMI - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NMI - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NMI - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NMI+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047293870445,"sku":"FY12595","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_88aa648e-9f59-483c-b8e6-97487c0de0c8.jpg?v=1772019313","url":"https:\/\/www.ebiohippo.com\/products\/nmi-antibody-n-myc-and-stat-interactor-bha17135498","provider":"BioHippo","version":"1.0","type":"link"}