{"product_id":"nnmt-antibody-nicotinamide-n-methyltransferase-bha17135274","title":"NNMT Antibody \/ Nicotinamide N-methyltransferase","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNNMT Antibody \/ Nicotinamide N-methyltransferase is a anti-NNMT Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NNMT\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC\/IF, IP, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eThe NNMT antibody targets Nicotinamide N-methyltransferase, a cytosolic enzyme encoded by the NNMT gene that catalyzes the methylation of nicotinamide to form 1-methylnicotinamide (1-MNA) using S-adenosyl-L-methionine (SAM) as a methyl donor. This enzyme plays an essential role in nicotinamide metabolism and methyl group utilization, linking energy homeostasis with epigenetic regulation. Nicotinamide N-methyltransferase influences cellular NAD+ turnover, methylation capacity, and energy balance. The NNMT antibody enables detailed investigation of this metabolic enzyme in physiology, cancer, and metabolic disease.\u003cbr\u003e\u003cbr\u003eNicotinamide N-methyltransferase is expressed in various tissues, with the highest levels in liver, adipose tissue, and skeletal muscle. By regulating nicotinamide levels, NNMT indirectly affects sirtuin activity and NAD+-dependent deacetylation pathways. The NNMT antibody supports research into how this enzyme modulates mitochondrial function, stress resistance, and cellular metabolism. Its detection across metabolic tissues allows mapping of expression patterns that correlate with nutrient availability and hormonal regulation.\u003cbr\u003e\u003cbr\u003eDysregulation of NNMT expression is associated with multiple diseases. Overexpression promotes tumorigenesis by altering cellular methyl donor availability and epigenetic modifications. Elevated Nicotinamide N-methyltransferase activity has been observed in cancers of the liver, kidney, and pancreas, where it contributes to proliferation and metastasis. Conversely, reduced NNMT activity has been linked to insulin resistance and altered lipid metabolism. The NNMT antibody is widely used to quantify enzyme levels and evaluate its role in metabolic reprogramming, energy balance, and disease progression.\u003cbr\u003e\u003cbr\u003eIn metabolic research, Nicotinamide N-methyltransferase is recognized as a master regulator of one-carbon metabolism, influencing the methionine cycle and SAM\/SAH ratio. The NNMT antibody enables functional studies exploring how modulation of enzyme activity impacts methylation reactions and gene expression. Additionally, NNMT-derived metabolites such as 1-MNA exhibit signaling functions that affect endothelial function and anti-inflammatory responses, broadening the enzyme's physiological relevance.\u003cbr\u003e\u003cbr\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NNMT - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NNMT - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NNMT - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NNMT - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NNMT+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047288758637,"sku":"FY12371","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_c0f8e948-40bf-4949-a02c-44fcdfe99b12.jpg?v=1772019284","url":"https:\/\/www.ebiohippo.com\/products\/nnmt-antibody-nicotinamide-n-methyltransferase-bha17135274","provider":"BioHippo","version":"1.0","type":"link"}