{"product_id":"nol9-antibody-nucleolar-protein-9-bha17135335","title":"NOL9 Antibody \/ Nucleolar protein 9","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNOL9 Antibody \/ Nucleolar protein 9 is a anti-NOL9 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NOL9\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC\/IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eThe NOL9 antibody targets Nucleolar protein 9, a multifunctional nucleolar protein encoded by the NOL9 gene. Nucleolar protein 9 participates in ribosomal RNA (rRNA) processing, specifically catalyzing the 5'-end maturation of 28S and 5.8S rRNA during large subunit biogenesis. It possesses polynucleotide 5'-kinase activity that promotes efficient pre-rRNA cleavage and processing. The NOL9 antibody enables researchers to study rRNA maturation, nucleolar organization, and ribosome assembly in proliferating cells.\u003cbr\u003e\u003cbr\u003eNucleolar protein 9 localizes to the granular component of the nucleolus, where late steps of ribosome biogenesis occur. It interacts with the Las1L complex, which couples endonucleolytic cleavage with phosphorylation of the pre-rRNA substrate. The NOL9 antibody supports detailed localization studies, showing characteristic punctate nucleolar staining consistent with ribosome assembly regions. Depletion of NOL9 disrupts pre-rRNA maturation and impairs large ribosomal subunit formation, leading to nucleolar stress and p53 activation.\u003cbr\u003e\u003cbr\u003eBeyond ribosome biogenesis, Nucleolar protein 9 has been linked to cell growth regulation and stress responses. It participates in pathways that maintain nucleolar integrity under conditions of transcriptional or metabolic stress. The NOL9 antibody allows researchers to assess these processes and to investigate how nucleolar function integrates with global gene-expression control. Its activity is upregulated during proliferation, reflecting the dependence of ribosome production on cellular growth signals.\u003cbr\u003e\u003cbr\u003eDisruption or downregulation of NOL9 has been observed in cancer and developmental disorders associated with defective ribosome synthesis. The NOL9 antibody provides a tool for analyzing expression patterns in tumor and normal tissues, supporting exploration of ribosome biogenesis as a therapeutic target. Elevated NOL9 expression correlates with high ribosomal demand in rapidly dividing cells, making it a marker of proliferative capacity.\u003cbr\u003e\u003cbr\u003eThe NOL9 antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, showing strong nucleolar localization.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NOL9 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NOL9 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NOL9 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NOL9 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NOL9+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047289446765,"sku":"FY12432","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_a5b5e39f-f9c7-42b4-bdf0-f12db89038fc.jpg?v=1782236998","url":"https:\/\/www.ebiohippo.com\/products\/nol9-antibody-nucleolar-protein-9-bha17135335","provider":"BioHippo","version":"1.0","type":"link"}